Linked Life Data

2817398

Source:http://linkedlifedata.com/resource/pubmed/id/2817398

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-11-27
pubmed:abstractText
A method for the measurement of S-adenosylmethionine in cerebrospinal fluid and brain using high-performance liquid chromatography with electrochemical detection is described. The method is based upon the catechol O-methyltransferase-catalyzed methylation of dihydroxybenzylamine to its 3- and 4-methoxy derivatives and measurement of the latter. Detection limits are 0.1 pmol for 3- and 4-methoxyhydroxybenzylamine, recovery is 100%, and variability is less than 8%. HPLC measurement of 3- and 4-methoxyhydroxybenzylamine is linear up to at least 100 pmol injected. The use of a nonbiological catechol in the assay minimizes interference from endogenous and exogenous catechols and the wide limits of linearity allows the assay of small volumes of cerebrospinal fluid or brain extract without further concentration or dilution.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:volume
181
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
331-5
pubmed:dateRevised
2009-9-29
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
A method for the measurement of S-adenosylmethionine in small volume samples of cerebrospinal fluid or brain using high-performance liquid chromatography-electrochemistry.
pubmed:affiliation
Department of Child Health, Institute of Child Health, London, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't