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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1989-8-25
|
| pubmed:abstractText |
The single gene for human macrophage colony-stimulating factor (M-CSF, or CSF-1) generates multiple mRNA species that diverge within the coding region. We have characterized translation products of these mRNA species from native and recombinant sources. Immunoblots of reduced native M-CSF indicate that multiple glycosylated species ranging from 25 kd to 200 kd are secreted by human monocytes and cell lines. In contrast, CV-1 cells expressing a short M-CSF clone secrete only 24 kd recombinant M-CSF. Synthetic peptide antibodies were developed to distinguish between secreted recombinant M-CSF from long and short mRNA splicing variants. Immunoblot analysis indicates that alternative mRNA splicing generates some M-CSF protein heterogeneity. Most secreted MIA PaCa-2 M-CSF reacts with long-clone-specific antibody. Lectin affinity chromatography shows that variable glycosylation contributes significantly to MIA PaCa-2 M-CSF size heterogeneity. In addition, cell lysates also contain larger M-CSF species that apparently undergo proteolytic processing before secretion. The data indicate that M-CSF protein heterogeneity results from both pre- and post-translational processing.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0730-2312
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
40
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
91-107
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2787329-Blotting, Western,
pubmed-meshheading:2787329-Chromatography, Affinity,
pubmed-meshheading:2787329-Colony-Stimulating Factors,
pubmed-meshheading:2787329-Glycosylation,
pubmed-meshheading:2787329-Humans,
pubmed-meshheading:2787329-Hydrolysis,
pubmed-meshheading:2787329-Macrophages,
pubmed-meshheading:2787329-RNA, Messenger,
pubmed-meshheading:2787329-RNA Splicing,
pubmed-meshheading:2787329-Radioimmunoassay,
pubmed-meshheading:2787329-Tumor Cells, Cultured
|
| pubmed:year |
1989
|
| pubmed:articleTitle |
Human macrophage colony-stimulating factor heterogeneity results from alternative mRNA splicing, differential glycosylation, and proteolytic processing.
|
| pubmed:affiliation |
Department of Protein Chemistry, Cetus Corporation, Emeryville, California 94608.
|
| pubmed:publicationType |
Journal Article
|