Linked Life Data

2782652

Source:http://linkedlifedata.com/resource/pubmed/id/2782652

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1989-10-18
pubmed:abstractText
Myocardial depressant action of local anesthetics at toxic concentrations is well documented. Their effect on vascular smooth muscle, however, remains controversial. This study analyzed local anesthetic action on the subcellular smooth-muscle contractile system. Highly purified smooth-muscle myosin and myosin light-chain kinase (MLCK) were prepared from fresh turkey gizzards, and the phosphorylation of the myosin light-chain in the presence of Ca++ and calmodulin was evaluated by the urea gel-electrophoresis. Tetracaine 1.3.10(-3) M and bupivacaine 7.5.10(-3) M inhibited the MLCK 50%, whereas lidocaine 2.5.10(-2) M inhibited the MLCK 40%. The inhibitory action was partially reversed by increasing the calcium ion concentration from 1.10(-5) to 2.10(-4) M. On the other hand, raising the calmodulin concentration from 1.10(-7) M to 6.10(-7) M completely reversed the inhibition. A possible cause of the discrepancy in the anesthetic concentrations between subcellular and in vivo studies is discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0003-2999
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
504-10
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Smooth muscle contraction and local anesthetics: calmodulin-dependent myosin light-chain kinase.
pubmed:affiliation
Department of Anesthesia, University of Utah School of Medicine, Salt Lake City.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.