Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1977-11-30
|
| pubmed:abstractText |
When [35S]cysteine was injected adjacent to the supraoptic nucleus (SON) in rats, it was rapidly incorporated into proteins in the SON. The [35S]cysteine-labeled proteins extracted from the SON were separated by isoelectric focusing on polyacrylamide gels. Twenty minutes after the injection of [35S]cysteine, two major labeled peaks (pI = 5.4 and 6.1) were found in the SON of normal rats; Brattleboro rats had only one major labeled peak (pI = 5.4). One hour after the injection, four major radioactive peaks were found in the SON of normal animals (pI = 5.1, 5.4, 5.6, and 6.1). Animals with diabetes insipidus had only two major labeled proteins (pI = 5.1 AND 5.4). Twenty-four hours after normal rats were injected with [35S]cysteine, all of the labeled peaks described above, except for the one with pI = 5.1, had decreased markedly in size and a small amount of labeled protein with pI about 4.8 was present in the SON. After 24 hr the posterior pituitary of normal animals contained two [35S]cysteine-labeled proteins with pI = 4.6 AND 4.8. The pituitaries of Brattleboro rats had only the pI = 4.6 labeled protein. These pulse-chase data, with data we have presented elsewhere, indicate that the vasopressin- and oxytocin-neurophysins are synthesized as parts of separate precursors (pI = 6.1 and 5.4, respectively). These precursors are converted into at least two intermediates (pI = 5.6 and 5.1) which, in turn, yield the vasopressin-neurophysin (pI = 4.8) and the oxytocin-neurophysin (pI = 4.6).
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/269451-1091193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/269451-1174948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/269451-14236104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/269451-14236105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/269451-14440609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/269451-180015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/269451-236308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/269451-4747772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/269451-4902949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/269451-5127429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/269451-65791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/269451-858741
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0027-8424
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
74
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4046-9
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:269451-Animals,
pubmed-meshheading:269451-Cysteine,
pubmed-meshheading:269451-Diabetes Insipidus,
pubmed-meshheading:269451-Female,
pubmed-meshheading:269451-Hypothalamus,
pubmed-meshheading:269451-Isoelectric Focusing,
pubmed-meshheading:269451-Molecular Weight,
pubmed-meshheading:269451-Neurophysins,
pubmed-meshheading:269451-Rats,
pubmed-meshheading:269451-Species Specificity,
pubmed-meshheading:269451-Supraoptic Nucleus
|
| pubmed:year |
1977
|
| pubmed:articleTitle |
Neurophysin biosynthesis in normal rats and in rats with hereditary diabetes insipidus.
|
| pubmed:publicationType |
Journal Article,
Comparative Study
|