Linked Life Data

2663704

Source:http://linkedlifedata.com/resource/pubmed/id/2663704

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-8-25
pubmed:abstractText
We have detected significant levels of IL-1, TNF and IL-6 in ascitic fluid from mice injected with different hybridomas. Each of these murine cytokines is active on a wide range of both human and murine target cells, and all are involved in inflammatory responses in vivo. We could not detect TNF or IL-1 in culture supernatant from the hybridoma line, used to generate the ascites; however, one out of ten did produce high levels of IL-6. These cytokines can interfere in many in vitro systems where monoclonal antibodies are used as diluted ascitic fluid to inhibit their ligands. A potentially more serious problem could arise with monoclonal antibodies used for therapy, in vivo diagnosis, or as ex vivo reagents. The use of monoclonal antibodies derived from culture supernatants is, therefore, obviously preferable to use of ascitic fluid for clinical applications, however, even with supernatant it cannot be assumed that cytokines are not present. Cytokine levels should, therefore, be monitored in all starting solutions and if present, their clearance during purification should be determined.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0272-457X
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
361-7
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Presence of the inflammatory cytokines IL-1, TNF, and IL-6 in preparations of monoclonal antibodies.
pubmed:affiliation
National Institute for Biological Standards and Control, South Mimms, Potters Bar, Herts.
pubmed:publicationType
Journal Article