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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1989-7-3
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pubmed:abstractText |
Pancreatic islet beta-cells and insulin-producing RINm5F cells were electroporated in the presence of the c-Ha-ras oncogene, to assess the possible involvement of the encoded product in coupling extracellular receptors to phospholipase C. After two days the c-Ha-ras-transfected cells increased their expression of c-Ha-ras mRNA. These cells were also found to contain more [3H]InsP3, suggesting an increased basal (non-ligand-activated) phospholipase C activity. In addition, the transfected cells were unable to respond to ligand (bombesin) activation of phospholipase C. The ras-transfected insulin-producing cells showed enhanced phosphorylation of a 200 kDa substrate crossreacting with an antibody to an 80 kDa protein kinase C substrate. The phorbol ester 12-O-tetradecanoyl 13-acetate and bombesin also induced phosphorylation of the 200 kDa substrate. All of these changes occurred without changes in the rates of [3H]thymidine incorporation. The results suggest that the mutated c-Ha-ras oncogene directly or indirectly stimulates the basal phospholipase C activity of these cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-13563465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-169123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-2438556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-2822028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-2829167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-2834200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-2842715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-2883654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-2986003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-2988501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-2990725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-3018591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-3026648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-3027568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-3097518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-3111466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-3112584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-3313065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-3325038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-3458242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-3487832,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-3546293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-3574471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-3680264,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-6318112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-6373015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-6601105,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/2658977-791956
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
259
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
701-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2658977-Animals,
pubmed-meshheading:2658977-Bombesin,
pubmed-meshheading:2658977-Calcium,
pubmed-meshheading:2658977-Genes, ras,
pubmed-meshheading:2658977-Islets of Langerhans,
pubmed-meshheading:2658977-Mice,
pubmed-meshheading:2658977-Phosphorylation,
pubmed-meshheading:2658977-Proteins,
pubmed-meshheading:2658977-Tetradecanoylphorbol Acetate,
pubmed-meshheading:2658977-Transfection,
pubmed-meshheading:2658977-Type C Phospholipases
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pubmed:year |
1989
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pubmed:articleTitle |
Transfection of insulin-producing cells with a transforming c-Ha-ras oncogene stimulates phospholipase C activity.
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pubmed:affiliation |
Department of Medical Cell Biology, Uppsala University, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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