| pubmed-article:2645281 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2645281 | lifeskim:mentions | umls-concept:C0024660 | lld:lifeskim |
| pubmed-article:2645281 | lifeskim:mentions | umls-concept:C0027882 | lld:lifeskim |
| pubmed-article:2645281 | lifeskim:mentions | umls-concept:C0006104 | lld:lifeskim |
| pubmed-article:2645281 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:2645281 | lifeskim:mentions | umls-concept:C0040688 | lld:lifeskim |
| pubmed-article:2645281 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:2645281 | lifeskim:mentions | umls-concept:C1527195 | lld:lifeskim |
| pubmed-article:2645281 | lifeskim:mentions | umls-concept:C1441616 | lld:lifeskim |
| pubmed-article:2645281 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:2645281 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:2645281 | pubmed:dateCreated | 1989-4-3 | lld:pubmed |
| pubmed-article:2645281 | pubmed:abstractText | In this communication, we demonstrate that adult mammalian brain neurons express transforming growth factor-alpha (TGF-alpha). We used the anti-TGF-alpha monoclonal antibody, MF9, to immunohistochemically localize TGF-alpha in human and rat brain. We found specific immunoreactivity in neurons throughout the brain which was not a result of cross-reactivity of MF9 with the neuropeptide, synenkephalin. Northern blot analysis of bovine and rat brain RNA using human and rat TGF-alpha cDNA probes, respectively, revealed a single 4.8-kilobase pair mRNA with approximately equal abundance in the bovine brainstem, cerebellum, hypothalamus, and cerebral cortex. Fetal rat brain had about 2-fold more TGF-alpha mRNA than did adult rat. The brain TGF-alpha cDNA was cloned from a human neonatal brainstem library. Four identical clones were isolated after screening 10(6) recombinant lambda gt11 phage. The sequence of the 894-base pair cDNA was virtually identical with the cDNA isolated from a human renal cell carcinoma. A single alanine codon was deleted in the brain cDNA at an exon-exon junction. The alanine deletion is within the amino-terminal region of the TGF-alpha precursor that is thought to be removed by proteolytic processing of the precursor to the mature growth factor. These studies indicate that the normal mammalian brain neurons express TGF-alpha. | lld:pubmed |
| pubmed-article:2645281 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2645281 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2645281 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2645281 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2645281 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2645281 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2645281 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:2645281 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:2645281 | pubmed:author | pubmed-author:KudlowJ EJE | lld:pubmed |
| pubmed-article:2645281 | pubmed:author | pubmed-author:PatersonA JAJ | lld:pubmed |
| pubmed-article:2645281 | pubmed:author | pubmed-author:LeungA WAW | lld:pubmed |
| pubmed-article:2645281 | pubmed:author | pubmed-author:AsaS LSL | lld:pubmed |
| pubmed-article:2645281 | pubmed:author | pubmed-author:KobrinM SMS | lld:pubmed |
| pubmed-article:2645281 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2645281 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:2645281 | pubmed:volume | 264 | lld:pubmed |
| pubmed-article:2645281 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2645281 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2645281 | pubmed:pagination | 3880-3 | lld:pubmed |
| pubmed-article:2645281 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:2645281 | pubmed:meshHeading | pubmed-meshheading:2645281-... | lld:pubmed |
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| pubmed-article:2645281 | pubmed:meshHeading | pubmed-meshheading:2645281-... | lld:pubmed |
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| pubmed-article:2645281 | pubmed:meshHeading | pubmed-meshheading:2645281-... | lld:pubmed |
| pubmed-article:2645281 | pubmed:year | 1989 | lld:pubmed |
| pubmed-article:2645281 | pubmed:articleTitle | Transforming growth factor-alpha in the mammalian brain. Immunohistochemical detection in neurons and characterization of its mRNA. | lld:pubmed |
| pubmed-article:2645281 | pubmed:affiliation | Department of Clinical Biochemistry, University of Toronto, Banting and Best Diabetes Centre, Ontario, Canada. | lld:pubmed |
| pubmed-article:2645281 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2645281 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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