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rdf:type |
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lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0007634,
umls-concept:C0013081,
umls-concept:C0015127,
umls-concept:C0018270,
umls-concept:C0067033,
umls-concept:C0237477,
umls-concept:C1314792,
umls-concept:C1511938,
umls-concept:C2717971,
umls-concept:C2745900
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pubmed:issue |
6
|
|
pubmed:dateCreated |
1990-2-7
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pubmed:databankReference |
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pubmed:abstractText |
Cells from the human leukemia cell line HL-60 undergo terminal differentiation when exposed to inducing agents. Differentiation of these cells is always accompanied by withdrawal from the cell cycle. Here we describe the isolation of a cDNA encoding a novel serine protease that is present in HL-60 cells and is down-regulated during induced differentiation of these cells. We have named this protease myeloblastin. Down-regulation of myeloblastin mRNA occurs with both monocytic and granulocytic inducers. Myeloblastin mRNA is undetectable in fully differentiated HL-60 cells as well as in human peripheral blood monocytes. We found that regulation of myeloblastin mRNA in HL-60 cells is serum dependent. Inhibition of myeloblastin expression by an antisense oligodeoxynucleotide inhibits proliferation and induces differentiation of promyelocyte-like leukemia cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0092-8674
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
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pubmed:pagination |
959-68
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2598267-Amino Acid Sequence,
pubmed-meshheading:2598267-Base Sequence,
pubmed-meshheading:2598267-Cell Differentiation,
pubmed-meshheading:2598267-Cell Division,
pubmed-meshheading:2598267-Cell Line,
pubmed-meshheading:2598267-Culture Media,
pubmed-meshheading:2598267-DNA, Neoplasm,
pubmed-meshheading:2598267-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:2598267-Genes,
pubmed-meshheading:2598267-Humans,
pubmed-meshheading:2598267-Leukemia, Promyelocytic, Acute,
pubmed-meshheading:2598267-Molecular Sequence Data,
pubmed-meshheading:2598267-Myeloblastin,
pubmed-meshheading:2598267-Oligodeoxyribonucleotides,
pubmed-meshheading:2598267-RNA, Messenger,
pubmed-meshheading:2598267-Serine Endopeptidases,
pubmed-meshheading:2598267-Transcription, Genetic,
pubmed-meshheading:2598267-Tumor Cells, Cultured
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pubmed:year |
1989
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pubmed:articleTitle |
Down-regulation of a serine protease, myeloblastin, causes growth arrest and differentiation of promyelocytic leukemia cells.
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pubmed:affiliation |
Department of Physiology and Cellular Biophysics, Columbia University College of Physicians and Surgeons, New York, New York 10032.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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