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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1989-12-27
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pubmed:abstractText |
Cas NS-1 is an acutely transforming murine retrovirus that induces early B-lineage lymphomas and occasional myeloid leukemias. The transforming sequence of this virus, v-cbl, shows no homology to known oncogenes but has some similarities to the yeast transcriptional factor GCN4. In this study we used a v-cbl probe to analyze mRNAs from a wide range of murine and human hemopoietic tumor cell lines and detected an 11-kilobase mRNA in all lineages. In normal mouse tissues the expression of c-cbl was highest in testis and thymus tissues, the predominant species in testis tissue being a 3.5-kilobase mRNA. The v-cbl oncogene was inserted into a bacterial expression vector to produce protein for the immunization of rabbits. Affinity-purified v-cbl antibodies identified abundant levels of p100gag-cbl in Cas NS-1-transformed fibroblasts and lower levels of a 135-kilodalton protein (p135c-cbl) in both normal and transformed cells. Subcellular fractionation showed that p100gag-cbl and p135c-cbl are both located in the nucleus and retained following 420 mM salt extraction. These results indicate that the translational product of a c-cbl is a 135-kilodalton nuclear protein.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-195075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-2760978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-2784003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-2965388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-3023837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-3047011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-3536236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-3600635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-3907852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-6090064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-6159641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-6289129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-6319013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-6548307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-6771761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2585608-6828386
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5420-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2585608-Animals,
pubmed-meshheading:2585608-Cell Line,
pubmed-meshheading:2585608-Cell Transformation, Neoplastic,
pubmed-meshheading:2585608-Gene Expression,
pubmed-meshheading:2585608-Genetic Vectors,
pubmed-meshheading:2585608-Hematopoietic Stem Cells,
pubmed-meshheading:2585608-Humans,
pubmed-meshheading:2585608-Leukemia,
pubmed-meshheading:2585608-Male,
pubmed-meshheading:2585608-Mice,
pubmed-meshheading:2585608-Nuclear Proteins,
pubmed-meshheading:2585608-Oncogene Protein v-cbl,
pubmed-meshheading:2585608-Organ Specificity,
pubmed-meshheading:2585608-Proto-Oncogenes,
pubmed-meshheading:2585608-RNA, Messenger,
pubmed-meshheading:2585608-Restriction Mapping,
pubmed-meshheading:2585608-Retroviridae Proteins, Oncogenic,
pubmed-meshheading:2585608-Testis,
pubmed-meshheading:2585608-Thymus Gland,
pubmed-meshheading:2585608-Transcription, Genetic
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pubmed:year |
1989
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pubmed:articleTitle |
The c-cbl proto-oncogene is preferentially expressed in thymus and testis tissue and encodes a nuclear protein.
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pubmed:affiliation |
Division of Human Immunology, Institute of Medical and Veterinary Science, Adelaide, South Australia.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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