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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1989-11-9
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pubmed:abstractText |
Synthesis of rRNA by RNA polymerase I is almost completely inhibited soon after infection of human cells with poliovirus. We show that extracts prepared from poliovirus-infected HeLa cells are severely inhibited in their ability to transcribe from a human rDNA promoter compared with extracts from mock-infected cells. Two lines of evidence presented here suggest that a specific transcriptional activity required for rDNA transcription in vitro is impaired in virus-infected cells. First, fractionation of individual transcriptional components by phosphocellulose chromatography and subsequent reconstitution experiments showed that the specific transcriptional activity of fraction C (0.8 M KCl eluate) from virus-infected cells was reduced three- to fourfold relative to that isolated from mock-infected cells. The activities of other transcription factors needed for in vitro transcription from the rDNA promoter were unaffected. Second, fraction C derived from mock-infected cells specifically restored transcription in extracts prepared from virus-infected cells. Fraction C contained both a nonspecific RNA polymerase I elongation activity and a specific factor activity which was needed for accurate transcription initiation. It is the specific transcriptional activity and not the nonspecific chain elongation activity of fraction C that is affected in cells infected with poliovirus.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-14003696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-14153526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-201639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-223155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-223165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-2419318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-2828918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-2850483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-3018486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-3033310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-3413483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-3527052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-3621348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-3708692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-3796586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-3796588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-3856225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-3929071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-4000974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-4022007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-4070001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-429265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-4350413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-4368675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-4371761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-4472266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-6086108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-6090561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-6193397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-6262766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-6322167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-6688417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-6700588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-7063022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-7153690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-7177852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2552146-7326749
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
63
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4689-96
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2552146-Cell Transformation, Viral,
pubmed-meshheading:2552146-DNA, Ribosomal,
pubmed-meshheading:2552146-HeLa Cells,
pubmed-meshheading:2552146-Humans,
pubmed-meshheading:2552146-Kinetics,
pubmed-meshheading:2552146-Plasmids,
pubmed-meshheading:2552146-Poliovirus,
pubmed-meshheading:2552146-RNA, Ribosomal,
pubmed-meshheading:2552146-RNA Polymerase I,
pubmed-meshheading:2552146-Transcription, Genetic,
pubmed-meshheading:2552146-Transcription Factors
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pubmed:year |
1989
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pubmed:articleTitle |
Inhibition of rRNA synthesis by poliovirus: specific inactivation of transcription factors.
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pubmed:affiliation |
Department of Microbiology and Immunology, UCLA School of Medicine.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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