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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1990-1-19
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pubmed:abstractText |
Since radioiodination of human granulocyte colony-stimulating factor (G-CSF) is difficult, we synthesized a mutein of human G-CSF that retains full biological activity and receptor-binding capacity for at least 2 weeks after radioiodination. Receptors for human G-CSF were characterized in the plasma membrane fraction from the human term placenta (human placental membranes) and trophoblastic cells by using the 125I-labeled mutein of human G-CSF (KW-2228). The specific binding of 125I-labeled KW-2228 to placental membranes was pH-dependent, with maximal specific binding at pH 7.8; it increased linearly with protein to 3.7 mg of protein per ml and was both time- and temperature-dependent, with maximal binding at 4 degrees C after a 24-hr incubation. When we examined the ability of hematopoietic growth factors to inhibit 125I-labeled KW-2228 binding, we found that KW-2228 and intact human G-CSF inhibited 125I-labeled KW-2228 binding, whereas erythropoietin or granulocyte-macrophage colony-stimulating factor did not. Scatchard analysis revealed a single receptor type with a Bmax of 210 fmol/mg of protein and a Kd of 480 pM. The human G-CSF receptors on human placental membranes were shown to consist of two molecular species of 150 kDa and 120 kDa that could be specifically cross-linked to 125I-labeled KW-2228. Human trophoblastic cells, T3M-3, also possessed a single receptor for G-CSF with a Bmax of 533 receptors per cell and a Kd of 390 pM. Thus we have identified the receptor for human G-CSF on human placental membranes and trophoblastic cells, and the presence of this receptor in these membranes suggests that human G-CSF plays some role in the feto-placental unit during human development.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-2420009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-2446141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-2447047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-2461195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-3004958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-3011859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-3017972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-3038212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-3095282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-313822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-3484805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-406329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-4361410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-6299435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-6308423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-6345543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-6603906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2480598-6978344
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9323-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2480598-Binding, Competitive,
pubmed-meshheading:2480598-Cell Line,
pubmed-meshheading:2480598-Cell Membrane,
pubmed-meshheading:2480598-Cloning, Molecular,
pubmed-meshheading:2480598-Colony-Stimulating Factors,
pubmed-meshheading:2480598-DNA,
pubmed-meshheading:2480598-Female,
pubmed-meshheading:2480598-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:2480598-Humans,
pubmed-meshheading:2480598-Kinetics,
pubmed-meshheading:2480598-Placenta,
pubmed-meshheading:2480598-Pregnancy,
pubmed-meshheading:2480598-Receptors, Cell Surface,
pubmed-meshheading:2480598-Receptors, Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:2480598-Trophoblasts
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pubmed:year |
1989
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pubmed:articleTitle |
Identification and characterization of receptors for granulocyte colony-stimulating factor on human placenta and trophoblastic cells.
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pubmed:affiliation |
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
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pubmed:publicationType |
Journal Article
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