Linked Life Data

2473792

Source:http://linkedlifedata.com/resource/pubmed/id/2473792

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-8-30
pubmed:abstractText
Purified preparations of recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte CSF (G-CSF), and interleukin 3 (IL-3 or multi-CSF) alone and in combination, have been compared for their stimulatory effects on human granulocyte-macrophage colony forming cells (GM-CFC). In cultures of unseparated normal human bone marrow, the combinations of G-CSF plus IL-3 and GM-CSF plus IL-3 stimulated additive numbers of GM colonies, while GM-CSF plus G-CSF stimulated greater than additive numbers of GM colonies, compared with the sum of the colony formation obtained with each factor alone. Cultures of unseparated bone marrow, harvested from patients four to six days after administration of 5-fluorouracil (5-FU), resulted in additive GM colony formation with GM-CSF plus G-CSF, GM-CSF plus IL-3, and G-CSF plus IL-3. In order to address the possibility of secondary factor involvement in the synergistic interaction of GM-CSF and G-CSF, CD33+/CD34+ colony forming cells were separated from normal and post FU marrow by two color fluorescence activated cell sorting. In cultures of CD33+/CD34+ cells the combination of GM-CSF plus G-CSF stimulated a synergistic increase in GM colonies while GM-CSF plus IL-3 stimulated additive numbers of colonies. These results suggest that GM-CSF, G-CSF, and IL-3 stimulate distinct populations of GM-CFC. Furthermore GM-CSF and G-CSF interact synergistically and this action is a direct effect on progenitor cells not stimulated by GM-CSF or G-CSF alone.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
110-4
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:2473792-Antigens, CD, pubmed-meshheading:2473792-Antigens, CD34, pubmed-meshheading:2473792-Antigens, Differentiation, pubmed-meshheading:2473792-Antigens, Differentiation, Myelomonocytic, pubmed-meshheading:2473792-Bone Marrow, pubmed-meshheading:2473792-Bone Marrow Cells, pubmed-meshheading:2473792-Cells, Cultured, pubmed-meshheading:2473792-Colony-Stimulating Factors, pubmed-meshheading:2473792-Drug Synergism, pubmed-meshheading:2473792-Fluorouracil, pubmed-meshheading:2473792-Granulocyte Colony-Stimulating Factor, pubmed-meshheading:2473792-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:2473792-Growth Substances, pubmed-meshheading:2473792-Hematopoiesis, pubmed-meshheading:2473792-Hematopoietic Stem Cells, pubmed-meshheading:2473792-Humans, pubmed-meshheading:2473792-Interleukin-3
pubmed:year
1989
pubmed:articleTitle
Action of interleukin-3, G-CSF, and GM-CSF on highly enriched human hematopoietic progenitor cells: synergistic interaction of GM-CSF plus G-CSF.
pubmed:affiliation
Department of Internal Medicine, University of Virginia, Charlottesville.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.