Linked Life Data

2470881

Source:http://linkedlifedata.com/resource/pubmed/id/2470881

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1989-6-28
pubmed:abstractText
Amylase synthesis in cultured pancreatic acinar cells was determined using an affinity adsorbent, alpha-glucohydrolase inhibitor--Sepharose 4B. This adsorbent exhibited a consistent binding capacity and was specific for amylase. To assess the effects of antecedent diet on amylase synthesis during culture, acinar cells from rats fed high fat (HF) or high carbohydrate (HC) diets for 7 d were cultured for 1-48 h in serum-free medium. Amylase activity remained significantly higher in cells from rats fed the HC diet than in cells from rats fed the HF diet through 24 h in culture, despite an overall decrease with time in culture. The relative synthesis of amylase [([3H]phenylalanine amylase/[3H]phenylalanine total protein) x 100] was also significantly higher in cells from HC-fed rats than in cells from HF-fed rats at isolation and remained higher during culture. The results demonstrate that these cultured acinar cells synthesize amylase in vitro and that the effect of diet on amylase activity and relative synthesis persists during culture.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-3166
pubmed:author
pubmed:issnType
Print
pubmed:volume
119
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
805-12
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1989
pubmed:articleTitle
Synthesis of amylase by cultured rat pancreatic acinar cells: effects of antecedent diet.
pubmed:affiliation
Department of Nutrition and Food Science, University of Arizona, Tucson 85721.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't