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pubmed-article:2468648pubmed:abstractTextThe role of exonuclease III and endonuclease IV in the repair of pyrimidine dimers in bacteriophage T4-infected Escherichia coli was examined. UV-irradiated T4 showed reduced survival when plated on an xth nfo double mutant but showed wild-type survival on either single mutant. T4 denV phage were equally sensitive when plated on wild-type E. coli or an xth nfo double mutant, suggesting that these endonucleases function in the same repair pathway as T4 pyrimidine dimer-DNA glycosylase. A uvrA mutant of E. coli in which the repair of pyrimidine dimers was dependent on the T4 denV gene carried on a plasmid was constructed. Neither an xth nor an nfo derivative of this strain was more sensitive than the parental strain to UV irradiation. We were unable to construct a uvrA xth nfo triple mutant. In addition, T4, which turns off the host UvrABC excision nuclease, showed reduced plating efficiency on an xth nfo double mutant.lld:pubmed
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pubmed-article:2468648pubmed:articleTitleRole of exonuclease III and endonuclease IV in repair of pyrimidine dimers initiated by bacteriophage T4 pyrimidine dimer-DNA glycosylase.lld:pubmed
pubmed-article:2468648pubmed:affiliationDepartment of Biological Sciences, State University of New York, Albany 12222.lld:pubmed
pubmed-article:2468648pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:2468648pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
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