Linked Life Data

2455571

Source:http://linkedlifedata.com/resource/pubmed/id/2455571

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-8-19
pubmed:abstractText
The effects of recombinant human erythropoietin (Ep), granulocyte/macrophage (GM) and granulocyte (G) colony-stimulating factors (CSF), and interleukin-3 (IL-3) on erythroid burst and GM colony growth have been studied in fetal bovine serum (FBS)-supplemented and FBS-deprived culture. Sources of progenitor cells were nonadherent or nonadherent T-lymphocyte-depleted marrow or peripheral blood cells from normal humans. G-CSF, in concentrations up to 2.3 X 10(-10) mol/L, induced only the formation of neutrophil colonies. In contrast, GM-CSF and IL-3 both induced GM colonies and sustained the formation of erythroid bursts in the presence of Ep. However, the activities of these growth factors were affected by the culture conditions. IL-3 induction of GM colonies depended on the presence of FBS, whereas the degree of GM-CSF induction of GM colonies in FBS-deprived cultures depended on the method by which adherent cells were removed. GM-CSF increased colony numbers in a concentration-dependent manner only if the cells had been prepared by overnight adherence. Both GM-CSF and IL-3 exhibited erythroid burst-promoting activity in FBS-deprived cultures. However, some lineage restriction was evident because GM-CSF was two- to threefold more active than IL-3 in inducing GM colonies but IL-3 was two- to threefold more active in promoting erythroid burst growth. Furthermore, in FBS-deprived cultures, the number of both erythroid bursts and GM colonies reached the maximum only when Ep, GM-CSF, and IL-3 or GM-CSF, IL-3, and G-CSF, respectively, were added together. These results suggest that the colonies induced by IL-3, GM-CSF, and G-CSF are derived from different progenitors.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
248-56
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:2455571-Bone Marrow Cells, pubmed-meshheading:2455571-Cell Adhesion, pubmed-meshheading:2455571-Cell Differentiation, pubmed-meshheading:2455571-Colony-Forming Units Assay, pubmed-meshheading:2455571-Colony-Stimulating Factors, pubmed-meshheading:2455571-Culture Media, pubmed-meshheading:2455571-Drug Combinations, pubmed-meshheading:2455571-Erythrocytes, pubmed-meshheading:2455571-Erythropoiesis, pubmed-meshheading:2455571-Erythropoietin, pubmed-meshheading:2455571-Fetal Blood, pubmed-meshheading:2455571-Granulocyte Colony-Stimulating Factor, pubmed-meshheading:2455571-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:2455571-Granulocytes, pubmed-meshheading:2455571-Growth Substances, pubmed-meshheading:2455571-Hematopoietic Stem Cells, pubmed-meshheading:2455571-Humans, pubmed-meshheading:2455571-Interleukin-3, pubmed-meshheading:2455571-Lymphocyte Depletion, pubmed-meshheading:2455571-Macrophages, pubmed-meshheading:2455571-Recombinant Proteins
pubmed:year
1988
pubmed:articleTitle
In vitro differentiation of human granulocyte/macrophage and erythroid progenitors: comparative analysis of the influence of recombinant human erythropoietin, G-CSF, GM-CSF, and IL-3 in serum-supplemented and serum-deprived cultures.
pubmed:affiliation
Department of Medicine, University of Washington, Seattle 98195.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't