Linked Life Data

2436752

Source:http://linkedlifedata.com/resource/pubmed/id/2436752

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1987-6-11
pubmed:abstractText
alpha-Difluoromethylornithine (DFMO) is an irreversible inhibitor of ornithine decarboxylase (ODC) and restricts the proliferation and differentiation of Friend murine erythroleukemia cells. We have studied the effect of this compound on the expression of ODC, transferrin receptor (TFR), and beta-globin during normal cellular proliferation and dimethylsulfoxide-induced terminal differentiation. Elevated RNA levels for ODC were observed during both normal Friend murine erythroleukemia cell division and replication associated with terminal differentiation, but these transcripts decreased as the cells ceased proliferating. However, in the presence of DFMO the levels of ODC remained elevated even when the cells had stopped dividing; this appears to be a feedback mechanism to overcome the effects of the inhibitor. TFR expression paralleled regular cell division and was curtailed when replication was reduced by DFMO. However, the inhibitor was unable to prevent the differentiation associated maintenance of TFR levels, well after proliferation terminated. While DFMO was able to restrict differentiation and hemoglobin synthesis, it did not inhibit the dimethylsulfoxide-induced expression of beta-globin RNA. We concluded that the block in differentiation caused by DFMO occurs along some pathway(s) other than the activation of beta-globin or TFR.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2638-44
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Altered expression of beta-globin, transferrin receptor, and ornithine decarboxylase in Friend murine erythroleukemia cells inhibited by alpha-difluoromethylornithine.
pubmed:publicationType
Journal Article