Linked Life Data

2428918

Source:http://linkedlifedata.com/resource/pubmed/id/2428918

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1986-11-12
pubmed:abstractText
Characteristic changes in the patterns of protein phosphorylation occur during meiotic maturation of mouse oocytes from the time subsequent to germinal vesicle breakdown, through metaphase II, and following fertilization. These changes occur during both in vitro or in vivo maturation or fertilization. Three major classes of changes in total phosphoprotein synthesis are observed. In the first class, protein phosphorylations increase from the germinal vesicle stage until just after germinal vesicle breakdown and then decrease during progression to metaphase II and after fertilization. The second class is characterized by decreases in protein phosphorylation during maturation with subsequent increases in phosphorylation of these proteins after fertilization. The third class is characterized by protein phosphorylations that remain relatively constant during maturation but increase after fertilization; phosphotyrosine phosphoproteins comprise the major species. The radiolabeled protein and phosphoprotein composition of isolated germinal vesicles was also examined, and a phosphoprotein of Mr 29,000 is found exclusively associated with the germinal vesicle. Since we have shown previously that 12-O-tetradecanoyl phorbol 13-acetate inhibits fertilization (Y.Endo, R.M. Schultz, and G.S. Kopf, submitted), we examined the effects of this compound on the phosphoprotein patterns of metaphase II eggs. 12-O-Tetradecanoyl phorbol 13-acetate treatment stimulates the phosphorylation of a specific phosphoprotein of Mr 80,000.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-104X
pubmed:author
pubmed:issnType
Print
pubmed:volume
239
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
401-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Stage-specific changes in protein phosphorylation accompanying meiotic maturation of mouse oocytes and fertilization of mouse eggs.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't