|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1986-6-27
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
Two different cDNAs for human granulocyte colony-stimulating factor (G-CSF) were isolated from a cDNA library constructed with mRNA prepared from human squamous carcinoma cells, which produce G-CSF constitutively. The nucleotide sequence analysis of both cDNAs indicated that two polypeptides coded by these cDNAs are different at one position where three amino acids are deleted/inserted. When the two cDNAs were introduced into monkey COS cells under the SV40 early promoter, both of them produced proteins having authentic G-CSF activity and some difference in the specific activity was suggested. A human gene library was then screened with the G-CSF cDNA and the DNA fragment containing the G-CSF chromosomal gene was characterized by the nucleotide sequence analysis. The human G-CSF gene is interrupted by four introns and a comparison of the structures of the two G-CSF cDNAs with that of the chromosomal gene indicated that the two mRNAs are generated by alternative use of two 5' splice donor sequences in the second intron of the G-CSF gene. When the G-CSF chromosomal gene was expressed in monkey COS cells by using the SV40 enhancer two mRNAs were detected by S1 mapping analysis.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-291033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-2983327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-2986009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-2990035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-2991860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-2996129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-301140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-313822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-322279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-3484805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-3856870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-3871523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-390497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-3923623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-3925454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-498284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6083453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6088074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6089176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6089345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6159536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6190815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6190911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6252477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6260373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6262811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6267479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6269091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6277502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6310323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6315415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6317187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6322187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6390681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6420702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6610831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6709041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6722880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6791577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-6929494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-7002232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-7063411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-7066982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-7226224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-728989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-728996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-7366736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2423327-822353
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0261-4189
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
5
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
575-81
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2423327-Humans,
pubmed-meshheading:2423327-Animals,
pubmed-meshheading:2423327-Kidney,
pubmed-meshheading:2423327-DNA,
pubmed-meshheading:2423327-Granulocytes,
pubmed-meshheading:2423327-Genes,
pubmed-meshheading:2423327-Carcinoma, Squamous Cell,
pubmed-meshheading:2423327-Base Sequence,
pubmed-meshheading:2423327-RNA, Messenger,
pubmed-meshheading:2423327-Amino Acid Sequence,
pubmed-meshheading:2423327-Chromosome Mapping,
pubmed-meshheading:2423327-Chromosomes, Human,
pubmed-meshheading:2423327-Cell Line,
pubmed-meshheading:2423327-Cercopithecus aethiops
|
