Linked Life Data

2413603

Source:http://linkedlifedata.com/resource/pubmed/id/2413603

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
1985-11-1
pubmed:abstractText
Aqueous suspensions of catalase crystals were freeze-cleaved, deep-etched and either shadowed with Ta/W at 45 degrees or decorated with 0.1-0.9 nm thick deposits of Au and Pt at normal incidence. The electron micrographs of the decorated specimens were processed by correlation averaging and compared with a relief reconstruction obtained from shadowed specimens. Pronounced decoration was observed on the catalase crystals at temperatures between 130 and 180 K. Disregarding the difficulties in interpretation, the averages of 0.1-0.2 nm thick Au and Pt films reveal more structural detail than the relief reconstruction. Perfect shadowing provides information on surface topography and is relatively easy to comprehend; decoration renders variations in physico-chemical affinity visible. Problems of the interference of decoration and shadowing effects in the intuitive interpretation of freeze-etch replicas and in relief reconstruction are discussed as well as the disturbing effects of a non-ideal carbon backing. The perspectives of using decoration intentionally as a positive staining technique for the investigation of frozen-hydrated surfaces are evaluated and quality criteria are defined.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0304-3991
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
305-20
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Decoration and shadowing of freeze-etched catalase crystals.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't