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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1990-6-26
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pubmed:databankReference | |
pubmed:abstractText |
The gene (npr) encoding an extracellular neutral metalloprotease (Npr) from Streptomyces cacaoi YM15 was cloned in Streptomyces lividans using pIJ702 as a vector. The nucleotide (nt) sequence of npr was determined. The deduced open reading frame encoded 550 amino acids (aa) (60 kDa) with a putative signal sequence of 34 aa at the N terminus. High-resolution S1 mapping identified the transcriptional start point at about 132-134 nt upstream from the start codon. The nt sequences at both -10 and -35 regions resemble the consensus sequence of typical Escherichia coli promoters and a fragment containing the promoter was functional in an E. coli promoter probe plasmid. In vitro transcription and translation of the cloned npr sequence revealed a 60-kDa protein product, correlated with the sequence data but not with the size (35 kDa) of the extracellular Npr. The N-terminal aa sequence in conjunction with the aa composition analyses on the purified mature Npr led to the conclusion that it was processed from the 60-kDa pre-proenzyme form encoded by npr. The Npr protease contained putative zinc ligand-binding regions and two repeated motifs, Asp-Ser-Gly, similar to the active site residues of the aspartic acid and retroviral proteases.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0378-1119
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
87-95
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2341042-Amino Acid Sequence,
pubmed-meshheading:2341042-Base Sequence,
pubmed-meshheading:2341042-Cloning, Molecular,
pubmed-meshheading:2341042-DNA Probes,
pubmed-meshheading:2341042-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2341042-Metalloendopeptidases,
pubmed-meshheading:2341042-Molecular Sequence Data,
pubmed-meshheading:2341042-Protein Biosynthesis,
pubmed-meshheading:2341042-Restriction Mapping,
pubmed-meshheading:2341042-Sequence Homology, Nucleic Acid,
pubmed-meshheading:2341042-Streptomyces,
pubmed-meshheading:2341042-Transcription, Genetic
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pubmed:year |
1990
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pubmed:articleTitle |
Extracellular metalloprotease gene of Streptomyces cacaoi: structure, nucleotide sequence and characterization of the cloned gene product.
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pubmed:affiliation |
Institute of Biochemistry, National Yang-Ming Medical College, Taipei, Taiwan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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