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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1990-5-4
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pubmed:abstractText |
Little is known about the non-antigen-specific signals delivered to T cells by dendritic cells (DC). Because several monocyte-derived factors like interleukins 1 alpha, 1 beta, and 6 (IL-1 alpha, IL-1 beta, and IL-6) enhance the T-cell proliferative responses, we studied the production of the above-mentioned cytokines by DC separated from human peripheral blood. The intracellular expression of the proteins (IL-1 alpha and IL-6) was studied at a single-cell level using an immunolabelling technique. The supernatants and cell lysates were studied with ELISA (IL-1 alpha and IL-1 beta). Northern blotting analysis was used to quantitate the mRNA levels. Several approaches were taken to stimulate the production of IL-1 alpha, IL-1 beta, and IL-6 by DC. These included the incubation of the DC in the presence of either LPS, rIL-1, or monoclonal anti-HLA-DR antibody, or the stimulation of cells with resting allogeneic T cells. None of the stimuli was able to induce the production of IL-1 alpha, IL-1 beta, or IL-6 by DC, whereas LPS-stimulated monocytes were strong producers of these mentioned cytokines and expressed the respective mRNA. Thus we concluded that IL-1 alpha, IL-1 beta, and IL-6 are primarily monocyte-derived factors and that these factors are not needed or produced during the activation of resting T cells by DC.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0300-9475
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
31
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
345-52
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2320953-Animals,
pubmed-meshheading:2320953-Cells, Cultured,
pubmed-meshheading:2320953-Dendritic Cells,
pubmed-meshheading:2320953-Humans,
pubmed-meshheading:2320953-Interleukin-1,
pubmed-meshheading:2320953-Interleukin-6,
pubmed-meshheading:2320953-Lipopolysaccharides,
pubmed-meshheading:2320953-Monocytes,
pubmed-meshheading:2320953-RNA, Messenger,
pubmed-meshheading:2320953-Rabbits
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pubmed:year |
1990
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pubmed:articleTitle |
Human peripheral blood-derived dendritic cells do not produce interleukin 1 alpha, interleukin 1 beta, or interleukin 6.
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pubmed:affiliation |
Department of Bacteriology and Immunology, University of Helsinki, Finland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|