| pubmed-article:2318859 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C0040669 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C0330390 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C2717940 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C1179435 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C0016006 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C0006556 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C1524075 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C1879748 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C1706853 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C1705248 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C1548799 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C1524073 | lld:lifeskim |
| pubmed-article:2318859 | lifeskim:mentions | umls-concept:C0449432 | lld:lifeskim |
| pubmed-article:2318859 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:2318859 | pubmed:dateCreated | 1990-5-10 | lld:pubmed |
| pubmed-article:2318859 | pubmed:abstractText | Previous studies indicated that synthesis of B beta chain may be a rate-limiting factor in the production of human fibrinogen since Hep G2 cells contain surplus pools of A alpha and gamma but not of B beta chains, and fibrinogen assembly commences by the addition of preformed A alpha and gamma chains to nascent B beta chains attached to polysomes. To test whether B beta chain synthesis is rate limiting Hep G2 cells were transfected with B beta cDNA, and its effect on fibrinogen synthesis and secretion was measured. Two sets of stable B beta cDNA-transfected Hep G2 cells were prepared, and both cell lines synthesized 3-fold more B beta chains than control cells. The B beta-transfected cells also synthesized and secreted increased amounts of fibrinogen. Transfection with B beta cDNA not only increased the synthesis of B beta chain but also increased the rate of synthesis of the other two component chains of fibrinogen and maintained surplus intracellular pools of A alpha and gamma chains. Transfection with B beta cDNA did not affect the synthesis of albumin, transferrin, or anti-chymotrypsin and had a small inhibitory effect on the synthesis of C-reactive protein. Taken together these studies demonstrate that increased B beta chain synthesis specifically causes increased production of the other two component chains of fibrinogen and that unequal and surplus amounts of A alpha and gamma chains are maintained intracellularly. | lld:pubmed |
| pubmed-article:2318859 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2318859 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2318859 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2318859 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:2318859 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2318859 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2318859 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:2318859 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2318859 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2318859 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2318859 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2318859 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2318859 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:2318859 | pubmed:author | pubmed-author:RoyS NSN | lld:pubmed |
| pubmed-article:2318859 | pubmed:author | pubmed-author:RedmanC MCM | lld:pubmed |
| pubmed-article:2318859 | pubmed:author | pubmed-author:MukhopadhyayG... | lld:pubmed |
| pubmed-article:2318859 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2318859 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:2318859 | pubmed:volume | 265 | lld:pubmed |
| pubmed-article:2318859 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2318859 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2318859 | pubmed:pagination | 6389-93 | lld:pubmed |
| pubmed-article:2318859 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2318859 | pubmed:meshHeading | pubmed-meshheading:2318859-... | lld:pubmed |
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| pubmed-article:2318859 | pubmed:meshHeading | pubmed-meshheading:2318859-... | lld:pubmed |
| pubmed-article:2318859 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2318859 | pubmed:articleTitle | Regulation of fibrinogen assembly. Transfection of Hep G2 cells with B beta cDNA specifically enhances synthesis of the three component chains of fibrinogen. | lld:pubmed |
| pubmed-article:2318859 | pubmed:affiliation | Lindsley F. Kimball Research Institute, New York Blood Center, New York 10021. | lld:pubmed |
| pubmed-article:2318859 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2318859 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2318859 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:2318859 | lld:pubmed |
