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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1991-2-20
|
| pubmed:abstractText |
By site-directed mutagenesis, Cys-116 was converted to Ser-116 in p-hydroxybenzoate hydroxylase (EC 1.14.13.2) from Pseudomonas fluorescens. In contrast to wild-type enzyme, the C116S mutant is no longer susceptible to oxidation by hydrogen peroxide and shows no reactivity towards 5,5'-dithiobis(2-nitrobenzoate). Crystals of the C116S mutant are isomorphous with the crystal form of wild-type enzyme. A difference electron density confirms the mutation made.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0014-5793
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
17
|
| pubmed:volume |
277
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
197-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2269354-4-Hydroxybenzoate-3-Monooxygenase,
pubmed-meshheading:2269354-Base Sequence,
pubmed-meshheading:2269354-Cloning, Molecular,
pubmed-meshheading:2269354-DNA Mutational Analysis,
pubmed-meshheading:2269354-Models, Molecular,
pubmed-meshheading:2269354-Molecular Sequence Data,
pubmed-meshheading:2269354-Oligonucleotides,
pubmed-meshheading:2269354-Protein Conformation,
pubmed-meshheading:2269354-Pseudomonas,
pubmed-meshheading:2269354-Sulfhydryl Reagents
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Engineering of microheterogeneity-resistant p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens.
|
| pubmed:affiliation |
Department of Biochemistry, Agricultural University, Wageningen, The Netherlands.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|