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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1979-10-17
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pubmed:abstractText |
1. It is confirmed that myosin light-chain kinase is a protein of mol.wt. about 80,000 that is inactive in the absence of calmodulin. 2. In the presence of 1 mol of calmodulin/mol of kinase 80-90% of the maximal activity is obtained. 3. Crude preparations of the whole light-chain fraction of rabbit fast-skeletal-muscle myosin contain enough calmodulin to activate the enzyme. A method for the preparation of calmodulin-free P light chain is described. 4. A procedure is described for the isolation of calmodulin from rabbit fast skeletal muscle. 5. Rabbit fast-skeletal-muscle calmodulin is indistinguishable from bovine brain calmodulin in its ability to activate myosin light-chain kinase. The other properties of these two proteins are also very similar. 6. Rabbit fast-skeletal-muscle troponin C was about 10% as effective as calmodulin as activator for myosin light-chain kinase. 7. By chromatography on a Sepharose-calmodulin affinity column evidence was obtained for the formation of a Ca2+-dependent complex between calmodulin and myosin light-chain kinase. 8. Troponin I from rabbit fast skeletal muscle and histone IIAS were phosphorylated by fully activated myosin light-chain kinase at about 1% of the rate of the P light chain.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-137127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-144501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-181375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-187190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-191460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-193860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-202300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-206540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-212300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-301524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-4254541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-4262960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-4349654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-435249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-4377105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-4776866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-4824205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-4853304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-5346361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-5550981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-561063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-5809510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-588245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-627540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/224861-628428
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
179
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
89-97
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
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pubmed:year |
1979
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pubmed:articleTitle |
Calmodulin and myosin light-chain kinase of rabbit fast skeletal muscle.
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pubmed:publicationType |
Journal Article
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