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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
|
pubmed:dateCreated |
1990-12-18
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pubmed:abstractText |
TNF plays a central role in septic shock induced by endotoxin or Gram-negative bacteria. Zymosan can elicit a septic shock-like syndrome in rodents in the absence of endotoxin. TNF and IL-6 release in mice treated with zymosan was investigated. One hour after intraperitoneal zymosan injection, maximal TNF levels were measured in serum, followed by IL-6 peak levels 1 h later. Treatment with a monoclonal antibody against TNF lowered zymosan-induced mortality from 63 to 11.6%, while maximal IL-6 levels were lowered by about 40%. Mechanisms triggering zymosan-induced cytokine release in murine macrophages were analysed in vitro. Cytokine release was only slightly triggered by uncoated zymosan particles. Thirty-nine per cent of TNF release by macrophages appeared to be triggered by zymosan-bound activated complement. Maximal TNF release also required the presence of natural antibodies against zymosan and zymosan-activated serum. In contrast, maximal IL-6 release was reached upon stimulation with zymosan-activated serum only, while the presence of zymosan particles lowered this response. We conclude that TNF is a crucial mediator in zymosan-induced shock. TNF release can be induced by different immunological pathways, without the need for the direct presence of endotoxins. Although IL-6 release during septic shock is partly dependent on TNF, in vitro trigger mechanisms for IL-6 and TNF differ remarkably.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
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pubmed:issn |
0300-9475
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
32
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
313-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2237286-Animals,
pubmed-meshheading:2237286-Antibodies, Monoclonal,
pubmed-meshheading:2237286-Complement Activation,
pubmed-meshheading:2237286-Disease Models, Animal,
pubmed-meshheading:2237286-Female,
pubmed-meshheading:2237286-Interleukin-6,
pubmed-meshheading:2237286-Macrophages,
pubmed-meshheading:2237286-Mice,
pubmed-meshheading:2237286-Peritoneal Cavity,
pubmed-meshheading:2237286-Random Allocation,
pubmed-meshheading:2237286-Shock, Septic,
pubmed-meshheading:2237286-Tumor Necrosis Factor-alpha,
pubmed-meshheading:2237286-Zymosan
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pubmed:year |
1990
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pubmed:articleTitle |
Tumour necrosis factor alpha (TNF-alpha) and interleukin 6 in a zymosan-induced shock model.
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pubmed:affiliation |
Department of Surgery, University of Limburg, Maastricht, The Netherlands.
|
pubmed:publicationType |
Journal Article,
In Vitro
|