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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1979-6-29
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pubmed:abstractText |
We analyzed large and small species of T-antigen by immunoprecipitation and two-dimensional gel electrophoresis. The T-antigen species were subjected to electrophoresis either directly or after reduction and alkylation with N-ethylmaleimide. Treatment with N-ethylmaleimide improved the resolution of large-T by two-dimensional gel electrophoresis and was a requirement for the resolution of small-t antigen on two dimensional gels. Large-T did not form a discrete protein spot, but rather formed a streak from approximately pH 6.5 to 6.9 on isoelectric focusing gels. Small-t formed a sharp protein spot at approximately pH 7.2 when subjected to electrophoresis under non-equilibrium conditions which extended the pH gradient to include proteins with basic isoelectric points. Treatment with N-ethylmaleimide decreased the mobility of the T-antigen species during sodium dodecyl sulfate gel electrophoresis. We suggest that the apparent increase in molecular weight was due to the association of N-ethylmaleimide with cysteine-rich regions of these proteins. Viable deletion mutants of simian virus 40 which do not induce the synthesis of small-t but product small-t-related polypeptides were used to localize the cysteine-rich region of small-t to between 0.54 and 0.59 on the genetic map of simian virus 40.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-1102604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-163374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-163375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-163376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-163377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-164665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-166203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-167188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-169100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-172906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-177974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-178902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-181749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-183010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-186787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-189036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-189091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-189092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-191809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-191834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-193255,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-198579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-203944,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-4288804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-4318787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-4372636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/219248-78572
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
587-96
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:219248-Alkylation,
pubmed-meshheading:219248-Antigens, Neoplasm,
pubmed-meshheading:219248-Antigens, Viral,
pubmed-meshheading:219248-Cell Line,
pubmed-meshheading:219248-Cell Transformation, Neoplastic,
pubmed-meshheading:219248-Ethylmaleimide,
pubmed-meshheading:219248-Isoelectric Point,
pubmed-meshheading:219248-Molecular Weight,
pubmed-meshheading:219248-Peptides,
pubmed-meshheading:219248-Simian virus 40
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pubmed:year |
1979
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pubmed:articleTitle |
Effect of alkylation on the physical properties of simian virus 40 T-antigen species.
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pubmed:publicationType |
Journal Article
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