Linked Life Data

2188143

Source:http://linkedlifedata.com/resource/pubmed/id/2188143

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-6-26
pubmed:abstractText
The localization of antigens and other substances in cells and tissues by electron microscopy is usually performed by immunohistochemical techniques employing labelled conventional or monoclonal antibodies. For the ultrastructural localization of the antibodies, they are coupled to electron-dense labels like gold or ferritin. Here, we demonstrate a novel method to localize antigens in cells, tissues, and on other supports. By electron energy loss spectroscopy (EELS) it is possible to directly analyze the distribution of antigens, metabolites or other substances without the use of labelled antibodies: as an example we demonstrate the distribution of the immunomodulator lipopeptide in B lymphocytes and macrophages. EELS represents a novel, sensitive, and generally applicable method for the detection and localization of antigens and other substances in biology and medicine.
pubmed:language
ger
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0028-1042
pubmed:author
pubmed:issnType
Print
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
110-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
[Electron energy loss spectroscopy (EELS) as a method for the localization of antigens and other substances in cells and tissues].
pubmed:affiliation
Arbeitsgruppe Medizinische Physik und Arbeitsgruppe Zelluläre Immunologie der Universität.
pubmed:publicationType
Journal Article, English Abstract, Review, Research Support, Non-U.S. Gov't