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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1990-12-11
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pubmed:abstractText |
Leukemic cells from a patient with Ph-negative chronic myeloid leukemia (CML) had a normal karyotype. M-BCR was rearranged and chromosome in situ hybridization showed an ABL insertion between 5' and 3' M-BCR on an apparently normal chromosome 22. The association of 5' BCR and 3' ABL at the 5' junction of the chromosome 9 insert was typical of that found for the BCR-ABL fusion gene in other patients with the standard t(9;22) and CML. With an M-bcr-3' probe, we cloned and characterized a 3' junction fragment. Field inversion gel electrophoresis and chromosome in situ hybridization studies using a probe isolated from genomic DNA 5' of the junction showed that 3' M-BCR was joined to a region of chromosome 9q34 rich in repetitive sequences and lying some distance 3' of ABL. The chromosome 9 insert was at least 329 kilobases long and included 3' ABL and a larger portion of chromosome 9q34. Our results allowed us to exclude transposon- or retroviral-mediated insertion of ABL into chromosome 22. Instead, we favored a two-translocation model in which a second translocation reconstituted a standard t(9;22)(q34;q11) but left the chromosome 9 insert, including 3' ABL, in chromosome 22.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
76
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pubmed:geneSymbol |
ABL,
M-BCR
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1812-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2171702-Chimera,
pubmed-meshheading:2171702-Chromosome Mapping,
pubmed-meshheading:2171702-Chromosomes, Human, Pair 22,
pubmed-meshheading:2171702-Chromosomes, Human, Pair 9,
pubmed-meshheading:2171702-DNA,
pubmed-meshheading:2171702-DNA Transposable Elements,
pubmed-meshheading:2171702-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2171702-Fusion Proteins, bcr-abl,
pubmed-meshheading:2171702-Genes, abl,
pubmed-meshheading:2171702-Humans,
pubmed-meshheading:2171702-Leukemia, Myeloid, Chronic, Atypical, BCR-ABL Negative,
pubmed-meshheading:2171702-Male,
pubmed-meshheading:2171702-Middle Aged,
pubmed-meshheading:2171702-Nucleic Acid Hybridization,
pubmed-meshheading:2171702-Protein-Tyrosine Kinases,
pubmed-meshheading:2171702-Proto-Oncogene Proteins,
pubmed-meshheading:2171702-Proto-Oncogene Proteins c-bcr,
pubmed-meshheading:2171702-Translocation, Genetic
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pubmed:year |
1990
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pubmed:articleTitle |
Ph-negative chronic myeloid leukemia: molecular analysis of ABL insertion into M-BCR on chromosome 22.
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pubmed:affiliation |
Department of Pathology, Children's Hospital of Los Angeles, CA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Case Reports,
Research Support, Non-U.S. Gov't
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