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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1990-11-19
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pubmed:abstractText |
Recombinant tumor necrosis factor-alpha (TNF-alpha) is a cytokine that induces proliferation of neoplastic B cells from patients with chronic lymphocytic leukemia (CLL). To gain insight into the mechanisms involved in regulating TNF responsiveness, we have examined TNF receptor expression on neoplastic B-CLL cells. We have demonstrated that freshly isolated neoplastic B cells from patients with CLL did not express TNF receptors. After 1 day of incubation in culture medium, TNF receptors were detectable in the range of 540 to 1,500/cell. Kinetic experiments revealed that receptor expression was half-maximal after 3 hours of culturing and required de novo protein synthesis. The Scatchard plots of TNF-alpha binding indicated a single set of high-affinity TNF receptors with a dissociation constant of 70 pmol/L. TNF receptor expression in vitro was found in all examined cases. All cytokines tested, with the exception of IL-2, did not influence the expression of TNF receptors. The TNF receptor expression is enhanced in B-CLL cells cultured in the presence of interleukin-2 when compared with the receptor expression of cells cultured in medium alone. Our data suggest that neoplastic B-CLL cells in patients with stable disease do not express TNF receptors in vivo and that an unknown mechanism suppressing TNF receptor expression in vivo may play a role in growth regulation of neoplastic B cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myc,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1607-13
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:2169926-B-Lymphocytes,
pubmed-meshheading:2169926-Cycloheximide,
pubmed-meshheading:2169926-Cytokines,
pubmed-meshheading:2169926-Gene Expression,
pubmed-meshheading:2169926-Humans,
pubmed-meshheading:2169926-Interleukin-2,
pubmed-meshheading:2169926-Kinetics,
pubmed-meshheading:2169926-Leukemia, Lymphocytic, Chronic, B-Cell,
pubmed-meshheading:2169926-Nucleic Acid Hybridization,
pubmed-meshheading:2169926-Protein Biosynthesis,
pubmed-meshheading:2169926-Proto-Oncogene Proteins,
pubmed-meshheading:2169926-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:2169926-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:2169926-RNA, Messenger,
pubmed-meshheading:2169926-Receptors, Cell Surface,
pubmed-meshheading:2169926-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:2169926-Recombinant Proteins,
pubmed-meshheading:2169926-Tumor Cells, Cultured,
pubmed-meshheading:2169926-Tumor Necrosis Factor-alpha
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pubmed:year |
1990
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pubmed:articleTitle |
Receptors for tumor necrosis factor on neoplastic B cells from chronic lymphocytic leukemia are expressed in vitro but not in vivo.
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pubmed:affiliation |
Department of Internal Medicine III, University of Ulm, FRG.
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pubmed:publicationType |
Journal Article,
Comparative Study
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