Source:http://linkedlifedata.com/resource/pubmed/id/21625016
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2011-5-31
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pubmed:abstractText |
Vitiligo is a pigmentation disorder in which inflammatory mediators such as cytokines have a pivotal role in disease's pathogenesis. Interleukin 17 (IL-17A) is a proinflammatory cytokine which is involved in the induction of several proinflamatory mediators such as cyclooxygenase 2 (COX2). The aim of this study was to investigate the gene expression of IL-17 and COX2 in peripheral blood leukocytes of vitiligo's patients.Peripheral blood leukocytes from 15 patients with vitiligo and 15 healthy controls were separated using a gradient density centrifugation method. After total RNA isolation and cDNA synthesis, IL-17 and COX2 gene expression were quantified by real-time polymerase chain reaction (PCR). There were no significant differences in IL-17 and COX2 gene expression in lymphocytes of patients with vitiligo compared with control group (p<0.05). However there was an increased IL-17 and COX2 gene expression in neutrophils of patients compared to controls, but it did not reach statistical significance (p=0.05). We could not find any differences in IL-17 and Cox2 gene expression between clinical diseases subtypes, sex and age. There was a significant correlation between IL-17 and COX2 genes expression in the neutrophils of patients (p=0.00, r=0.80). Our results showed an increased expression in IL-17 and Cox-2 genes in neurophils of patients with vitiligo. This suggested that these two factors are involved in the inflammatory process. Further studies with a larger sample size might help to establish the role of these factors in the pathogenesis of diseases.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/IL17A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-17,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1735-1502
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
81-9
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pubmed:meshHeading |
pubmed-meshheading:21625016-Adolescent,
pubmed-meshheading:21625016-Adult,
pubmed-meshheading:21625016-Child,
pubmed-meshheading:21625016-Cyclooxygenase 2,
pubmed-meshheading:21625016-Female,
pubmed-meshheading:21625016-Gene Expression Regulation,
pubmed-meshheading:21625016-Humans,
pubmed-meshheading:21625016-Interleukin-17,
pubmed-meshheading:21625016-Leukocytes,
pubmed-meshheading:21625016-Lymphocytes,
pubmed-meshheading:21625016-Male,
pubmed-meshheading:21625016-Middle Aged,
pubmed-meshheading:21625016-Neutrophils,
pubmed-meshheading:21625016-Polymerase Chain Reaction,
pubmed-meshheading:21625016-RNA, Messenger,
pubmed-meshheading:21625016-Vitiligo
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pubmed:year |
2011
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pubmed:articleTitle |
Expression of IL-17 and COX2 gene in peripheral blood leukocytes of vitiligo patients.
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pubmed:affiliation |
Rheumatic Disease Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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