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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2011-5-30
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pubmed:abstractText |
Ischemia is the most important factor that affects organ survival during harvesting. The two-layer method (TLM) is one of several cold storage solutions that seeks to preserve organs and cells avoiding in vivo and in vitro ischemia. We compared the retrieval of beta-like elements from exocrine pancreatic cells using TLM versus University of Wisconsin (UW) solutions. For this purpose pancreata laparoscopically harvested from 20 female pigs were preserved in UW solution or TLM before digestion. The resulting exocrine cells were divided into 2 groups: the first was cultured in a designed medium to allow differentiation into beta-like cells and the second was cryopreserved before the differentiation process at -196 °C for 8 weeks before culture in the same medium. The results revealed that TLM was better than UW as a preservation solution in terms of beta-cell viability and insulin secretion. We suggest that the use of TLM solution allows one to obtain less damaged cells for research purposes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Allopurinol,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorocarbons,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Organ Preservation Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygen,
http://linkedlifedata.com/resource/pubmed/chemical/Raffinose,
http://linkedlifedata.com/resource/pubmed/chemical/University of...
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1873-2623
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2011 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1173-7
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:21620081-Adenosine,
pubmed-meshheading:21620081-Allopurinol,
pubmed-meshheading:21620081-Animals,
pubmed-meshheading:21620081-Cell Differentiation,
pubmed-meshheading:21620081-Cell Separation,
pubmed-meshheading:21620081-Cell Survival,
pubmed-meshheading:21620081-Cells, Cultured,
pubmed-meshheading:21620081-Cryopreservation,
pubmed-meshheading:21620081-Culture Media,
pubmed-meshheading:21620081-Female,
pubmed-meshheading:21620081-Fluorocarbons,
pubmed-meshheading:21620081-Glucose,
pubmed-meshheading:21620081-Glutathione,
pubmed-meshheading:21620081-Insulin,
pubmed-meshheading:21620081-Insulin-Secreting Cells,
pubmed-meshheading:21620081-Laparoscopy,
pubmed-meshheading:21620081-Organ Preservation Solutions,
pubmed-meshheading:21620081-Oxygen,
pubmed-meshheading:21620081-Pancreas, Exocrine,
pubmed-meshheading:21620081-Pancreatectomy,
pubmed-meshheading:21620081-Raffinose,
pubmed-meshheading:21620081-Swine,
pubmed-meshheading:21620081-Time Factors,
pubmed-meshheading:21620081-Tissue and Organ Harvesting
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pubmed:year |
2011
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pubmed:articleTitle |
A good breath of oxygen for beta-like cells obtained from porcine exocrine pancreatic tissue.
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pubmed:affiliation |
Dipartimento di Discipline Chirurgiche ed Oncologiche, Università degli Studi di Palermo, A. Mirri, Palermo, Italy.
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pubmed:publicationType |
Journal Article,
Comparative Study
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