Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2011-4-22
pubmed:abstractText
The males of sex-linkaged balanced lethal silkworm strain (S-14) has two non-allelic recessive genes (lethal gene 1, l1 and lethal gene 2, l2). The two genes are located on two different Z chromosomes and cause death of embryos at body pigmentation stage and end reversal embryo stage, respectively. We firstly hybridized the males of S-14 strain with the females having wild-type genes of P50 strain and then backcrossed the males of F1 with females of P50 strain. A total of 1660 female moths of BC1 generation were divided into two groups, 1100 in BC1-l1 and 560 in BC1-l2 according to the lethal gene carried by these female moths' fathers-fame moths of F1, respectively. Based on the nucleotide sequence information from the published physical map of Bombyx mori, we developed 16 polymorphic SSR markers in l1 gene region and 18 polymorphic SSR makers in l2 gene region compared to the allelic region of P50 strain and used these SSR markers and groups of BC1-l1 and BC1-12 to map the two lethal genes, respectively. Gene l1 was mapped on the region of Z chromosome, covering a physical distance of 2.60 Mb. Gene l2 was fine mapped on the region of Z chromosome, covering a physical distance of 0.69 Mb.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0253-9772
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1269-74
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
[Mapping of the lethal genes in the sex-linkaged balanced lethal silkworm Bombyx mori using SSR markers].
pubmed:affiliation
Sericultural Research Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. xn0204@163.com
pubmed:publicationType
Journal Article, English Abstract