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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1990-10-18
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pubmed:databankReference |
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pubmed:abstractText |
Carotenoid biosynthesis is regulated by blue light during growth of Neurospora crassa mycelia. We have cloned the al-1 gene of N. crassa encoding the carotenoid-biosynthetic enzyme phytoene dehydrogenase and present an analysis of its structure and regulation. The gene encodes a 595-residue polypeptide that shows homology to two procaryotic carotenoid dehydrogenases. RNA measurements showed that the level of al-1 mRNA increased over 70-fold in photoinduced mycelia. Transcription run-on studies indicated that the al-1 gene was regulated at the level of initiation of transcription in response to photoinduction. The photoinduced increase of al-1 mRNA levels was not observed in two Neurospora mutants defective in all physiological photoresponses. Analysis of cosmid containing al-1 and of a translocation strain with a breakpoint within al-1 indicated that al-1 transcription proceeds towards the centromere of linkage group I of N. crassa.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-16593723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-16661992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-2527354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-2546948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-2656356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-2747617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-2868891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-2946938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-3005109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-3020015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-3293927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-3582984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-4270986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-6219280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-6220417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-6230518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-6235151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-6235211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-6269864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-6312838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-6340125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-6454899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-6546423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2144609-7358679
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5064-70
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:2144609-Amino Acid Sequence,
pubmed-meshheading:2144609-Base Sequence,
pubmed-meshheading:2144609-Cloning, Molecular,
pubmed-meshheading:2144609-Gene Expression Regulation, Fungal,
pubmed-meshheading:2144609-Genes, Fungal,
pubmed-meshheading:2144609-Light,
pubmed-meshheading:2144609-Molecular Sequence Data,
pubmed-meshheading:2144609-Neurospora,
pubmed-meshheading:2144609-Neurospora crassa,
pubmed-meshheading:2144609-Oxidoreductases,
pubmed-meshheading:2144609-RNA, Fungal,
pubmed-meshheading:2144609-RNA, Messenger,
pubmed-meshheading:2144609-Time Factors,
pubmed-meshheading:2144609-Transcription, Genetic
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pubmed:year |
1990
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pubmed:articleTitle |
Cloning, sequence, and photoregulation of al-1, a carotenoid biosynthetic gene of Neurospora crassa.
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pubmed:affiliation |
Department of Biological Sciences, Stanford University, California 94305-5020.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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