Linked Life Data

21444683

Source:http://linkedlifedata.com/resource/pubmed/id/21444683

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2011-4-5
pubmed:databankReference
pubmed:abstractText
To maintain tissue architecture, epithelial cells divide in a planar fashion, perpendicular to their main polarity axis. As the centrosome resumes an apical localization in interphase, planar spindle orientation is reset at each cell cycle. We used three-dimensional live imaging of GFP-labeled centrosomes to investigate the dynamics of spindle orientation in chick neuroepithelial cells. The mitotic spindle displays stereotypic movements during metaphase, with an active phase of planar orientation and a subsequent phase of planar maintenance before anaphase. We describe the localization of the NuMA and LGN proteins in a belt at the lateral cell cortex during spindle orientation. Finally, we show that the complex formed of LGN, NuMA, and of cortically located G?i subunits is necessary for spindle movements and regulates the dynamics of spindle orientation. The restricted localization of LGN and NuMA in the lateral belt is instructive for the planar alignment of the mitotic spindle, and required for its planar maintenance.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1540-8140
pubmed:author
pubmed:issnType
Electronic
pubmed:day
4
pubmed:volume
193
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
141-54
pubmed:dateRevised
2011-10-4
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
A lateral belt of cortical LGN and NuMA guides mitotic spindle movements and planar division in neuroepithelial cells.
pubmed:affiliation
Université de la Méditerranée/Aix-Marseille II, Developmental Biology Institute (IBDML), Centre National de la Recherche Scientifique UMR6216, Case 907, Campus de Luminy, 13288 Marseille, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't