Linked Life Data

21431708

Source:http://linkedlifedata.com/resource/pubmed/id/21431708

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-3-24
pubmed:abstractText
MicroRNAs (miRNAs) are noncoding RNA molecules that have come to attract considerable interest for their roles in animal and plant development and disease. One means to study miRNA function in animal development is to create mutations. Use of gene-targeting strategies based on ends-out homologous recombination is a useful approach to produce mutations of desired structure, and is gaining popularity for producing miRNA knockouts. Here we present a detailed protocol for miRNA gene targeting and for their subsequent molecular characterization as well as confirmation by rescue. The descriptions of a series of modified vectors designed to facilitate the analysis of miRNA function are included, and a method to manipulate the mutant genome using recombinase-mediated cassette exchange.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
732
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
99-120
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Protocols for use of homologous recombination gene targeting to produce microRNA mutants in Drosophila.
pubmed:affiliation
Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore. yawen@tll.org.sg
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't