|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1990-4-17
|
|
pubmed:abstractText |
To investigate the repertoire of autoantibodies in humans, anti-DNA and rheumatoid factor (RF) production in vitro was assessed in cultures of adult peripheral blood B cells and neonatal umbilical venous blood B cells. B cells were stimulated under various culture conditions, using an immobilized monoclonal anti-CD3 antibody and adult T cells or Staphylococcus aureus (SA) in the presence or absence of adult T cells or factors derived from mitogen-stimulated adult T cells as polyclonal B cell activators. Total IgM, as well as IgM anti-DNA and RF, were assessed by ELISA. Total IgM production was induced from adult and neonatal B cells with SA plus T cell factors, as well as anti-CD3-stimulated T cells. RF was induced from adult and cord blood B cells by either mode of stimulation, whereas significant anti-DNA production was observed only when B cells were stimulated with anti-CD3-activated T cells. These results confirm the presence of B cell precursors for autoantibodies in the preimmune as well as normal adult repertoire, and indicate that the production of anti-DNA and RF appears to be regulated independently.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-159322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-2452835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-2453555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-2579097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-2848890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-2967324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-302276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-3488857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-3495004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-3495631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-3511088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-3541943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-3655363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-3917274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-4038410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-6182766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-6249850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-6257787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-6299754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-6307568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-6409187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-6456303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-6458551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-6458553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-6470143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-6601551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-6983913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-7035553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2138166-7198664
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Antinuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Rheumatoid Factor
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0021-9738
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
85
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
899-903
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2138166-Adult,
pubmed-meshheading:2138166-Antibodies, Antinuclear,
pubmed-meshheading:2138166-Antigens, CD3,
pubmed-meshheading:2138166-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:2138166-B-Lymphocytes,
pubmed-meshheading:2138166-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:2138166-Female,
pubmed-meshheading:2138166-Fetal Blood,
pubmed-meshheading:2138166-Humans,
pubmed-meshheading:2138166-Immunoglobulin M,
pubmed-meshheading:2138166-Pregnancy,
pubmed-meshheading:2138166-Receptors, Antigen, T-Cell,
pubmed-meshheading:2138166-Rheumatoid Factor,
pubmed-meshheading:2138166-T-Lymphocytes
|
|
pubmed:year |
1990
|
|
pubmed:articleTitle |
In vitro autoantibody production by normal adult and cord blood B cells.
|
|
pubmed:affiliation |
Medical Service, Durham Veterans Administration Hospital, North Carolina 27705.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|
