Source:http://linkedlifedata.com/resource/pubmed/id/21316198
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2011-4-11
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| pubmed:abstractText |
The aim of this work was to investigate the molecular epidemiology and mechanisms responsible for reduced susceptibility to amoxicillin/clavulanic acid (AMC) amongst cefazolin-susceptible Klebsiella pneumoniae isolates from patients admitted to a chronic care institution. In total, 51 (29.8%) of 171 K. pneumoniae isolates recovered between 2006 and 2008 were non-susceptible to AMC, of which 45 were susceptible to cefazolin. Nucleotide sequencing analysis revealed that 19 produced IRT-11 and the remaining 26 were OXA-1-producers. All of the OXA-1-producing isolates harboured the aac(6')-Ib-cr-bla(OXA-1) cassette array, which in 23 isolates was located together with catB3 and arr3 within a class 1 integron and associated with qnrS2 (in 3 cases the integron lacked the qacE?1 and sul1 or sul3 genes). Genotyping analysis performed by enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) identified three different patterns amongst IRT-11-producing isolates (E1 to E3), with E1 being the most prevalent (63.2%), whilst the OXA-1-producing isolates were assigned to patterns E3 and E3a (isolates carrying typical class 1 integrons), E4 (isolates carrying defective integrons) and E5 (isolates without integrons). Genes encoding IRT-11 and OXA-1 were transferred by conjugation, and aac(6')-Ib-cr and qnrS2 were systematically co-transferred with bla(OXA-1). These results demonstrate that the high prevalence of decreased susceptibility to AMC amongst K. pneumoniae isolates from a chronic care hospital was mainly due to the simultaneous spread of two different clones, one of which comprised isolates producing IRT-11 and the other one comprised isolates that had acquired either the bla(OXA-1) gene located in a class 1 integron and linked to qnrS2 or the bla(IRT-11) gene.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1872-7913
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright © 2011 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
37
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
462-6
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| pubmed:meshHeading |
pubmed-meshheading:21316198-Amoxicillin-Potassium Clavulanate Combination,
pubmed-meshheading:21316198-Anti-Bacterial Agents,
pubmed-meshheading:21316198-Bacterial Typing Techniques,
pubmed-meshheading:21316198-Conjugation, Genetic,
pubmed-meshheading:21316198-Cross Infection,
pubmed-meshheading:21316198-DNA, Bacterial,
pubmed-meshheading:21316198-Drug Resistance, Bacterial,
pubmed-meshheading:21316198-Gene Order,
pubmed-meshheading:21316198-Genes, Bacterial,
pubmed-meshheading:21316198-Genotype,
pubmed-meshheading:21316198-Health Facilities,
pubmed-meshheading:21316198-Humans,
pubmed-meshheading:21316198-Integrons,
pubmed-meshheading:21316198-Klebsiella Infections,
pubmed-meshheading:21316198-Klebsiella pneumoniae,
pubmed-meshheading:21316198-Long-Term Care,
pubmed-meshheading:21316198-Microbial Sensitivity Tests,
pubmed-meshheading:21316198-Molecular Epidemiology,
pubmed-meshheading:21316198-Molecular Typing,
pubmed-meshheading:21316198-Polymerase Chain Reaction,
pubmed-meshheading:21316198-Sequence Analysis, DNA
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| pubmed:year |
2011
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| pubmed:articleTitle |
Molecular epidemiology and resistance mechanisms involved in reduced susceptibility to amoxicillin/clavulanic acid in Klebsiella pneumoniae isolates from a chronic care centre.
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| pubmed:affiliation |
Servei Anàlisis Clíniques, Hospital de Tortosa Verge de la Cinta, C/Esplanetes s/n 43500 Tortosa, Spain. marolgap@terra.es
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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