Linked Life Data

21296557

Source:http://linkedlifedata.com/resource/pubmed/id/21296557

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-2-21
pubmed:databankReference
pubmed:abstractText
Stenotrophomonas maltophilia is becoming a more and more common cause of infections. In this study, the minimal inhibitory concentrations of trimethoprim/sulfamethoxazole (SXT), ceftazidime, minocycline, levofloxacin, chloramphenicol and ticarcillin/clavulanic acid were determined and the distribution of integrons and sul1, sul2 and dfrA genes was investigated in 102 S. maltophilia isolates collected from patients treated in 31 hospitals in Anhui, China, in the month of September in 2006-2008. The rate of resistance to SXT was up to 30.4%, and 64.7% of isolates were class 1 integron-positive. Sequencing data revealed the following novel gene cassettes embedded in class 1 integrons: dfrA17-aadA5; dfrA12-aadA2; aacA4-catB8-aadA1; aadB-aac(6')-II-bla(CARB-8); and arr-3-aacA4. This is the first report of the gene cassettes dfrA17-aadA5 and dfrA12-aadA2 and of sul2 genes in SXT-resistant S. maltophilia isolates in China. None of the SXT-susceptible S. maltophilia isolates were positive for sul2 or dfrA gene products by polymerase chain reaction (PCR), but PCR products for sul1 were detected in 27 SXT-susceptible and 25 SXT-resistant isolates. The findings from this study indicate that the sul1 gene, in combination with dfrA17 and dfrA12 gene cassettes and sul2 genes located within a 7.3kb plasmid, lead to a high rate of SXT resistance and also confirm the need for ongoing resistance surveillance.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1872-7913
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
pubmed:issnType
Electronic
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
230-4
pubmed:meshHeading
pubmed-meshheading:21296557-Anti-Bacterial Agents, pubmed-meshheading:21296557-Bacterial Proteins, pubmed-meshheading:21296557-Base Sequence, pubmed-meshheading:21296557-Carrier Proteins, pubmed-meshheading:21296557-China, pubmed-meshheading:21296557-Cross Infection, pubmed-meshheading:21296557-Drug Resistance, Bacterial, pubmed-meshheading:21296557-Genes, Bacterial, pubmed-meshheading:21296557-Gram-Negative Bacterial Infections, pubmed-meshheading:21296557-Humans, pubmed-meshheading:21296557-Integrons, pubmed-meshheading:21296557-Microbial Sensitivity Tests, pubmed-meshheading:21296557-Molecular Sequence Data, pubmed-meshheading:21296557-Plasmids, pubmed-meshheading:21296557-Polymerase Chain Reaction, pubmed-meshheading:21296557-Sequence Analysis, DNA, pubmed-meshheading:21296557-Stenotrophomonas maltophilia, pubmed-meshheading:21296557-Trimethoprim-Sulfamethoxazole Combination
pubmed:year
2011
pubmed:articleTitle
Stenotrophomonas maltophilia resistance to trimethoprim/sulfamethoxazole mediated by acquisition of sul and dfrA genes in a plasmid-mediated class 1 integron.
pubmed:affiliation
Department of Infectious Diseases, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't