Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1991-9-19
pubmed:abstractText
Domoic acid is extracted from mussel tissue using the Association of Official Analytical Chemists procedure for paralytic shellfish toxins. This involves boiling the sample for 5 min with O.1N HCl then cooling and centrifuging. An aliquot of the supernatant is diluted 10 to 100 times with water, filtered and analyzed by reversed-phase liquid chromatography with a mobile phase of acetonitrile-water (12:88) at pH 2.5 and absorption detection at 242 nm. The detection limit is about 1 mg/kg domoic acid in seafood samples. The method was successfully used in collaborative studies and a survey of 44 different commercially purchased shellfish products from areas outside of Prince Edward Island showed no domoic acid greater than 1 mg/kg. The same method was applied to urine and feces from monkeys and blood (serum) from humans. The method was unsuccessful for urine and blood which required additional cleanup before analysis. The method worked well for feces at domoic acid levels greater than 1 mg/kg.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0382-232X
pubmed:author
pubmed:issnType
Print
pubmed:volume
16 Suppl 1E
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
27-31
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Determination of domoic acid in seafoods and in biological tissues and fluids.
pubmed:publicationType
Journal Article