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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2011-3-1
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pubmed:abstractText |
SynArfGEF, also known as BRAG3 or IQSEC3, is a member of the brefeldin A-resistant Arf-GEF/IQSEC family and was originally identified by screening for mRNA species associated with the post-synaptic density fraction. In this study, we demonstrate that synArfGEF activates Arf6, using Arf pull down and transferrin incorporation assays. Immunohistochemical analysis reveals that synArfGEF is present in somata and dendrites as puncta in close association with inhibitory synapses, whereas immunoelectron microscopic analysis reveals that synArfGEF localizes preferentially at post-synaptic specializations of symmetric synapses. Using yeast two-hybrid and pull down assays, we show that synArfGEF is able to bind utrophin/dystrophin and S-SCAM/MAGI-2 scaffolding proteins that localize at inhibitory synapses. Double immunostaining reveals that synArfGEF co-localizes with dystrophin and S-SCAM in cultured hippocampal neurons and cerebellar cortex, respectively. Both ?-dystroglycan and S-SCAM were immunoprecipitated from brain lysates using anti-synArfGEF IgG. Taken together, these findings suggest that synArfGEF functions as a novel regulator of Arf6 at inhibitory synapses and associates with the dystrophin-associated glycoprotein complex and S-SCAM.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADP-Ribosylation Factors,
http://linkedlifedata.com/resource/pubmed/chemical/ADP-ribosylation factor 6,
http://linkedlifedata.com/resource/pubmed/chemical/Acvrinp1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Dystrophin,
http://linkedlifedata.com/resource/pubmed/chemical/Guanine Nucleotide Exchange Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Guanylate Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/MAGI2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Magi2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vesicular Inhibitory Amino Acid...,
http://linkedlifedata.com/resource/pubmed/chemical/Viaat protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1471-4159
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pubmed:author |
pubmed-author:FukayaMasahiroM,
pubmed-author:HaraYoshinobuY,
pubmed-author:HarveyRobert JRJ,
pubmed-author:HataYutakaY,
pubmed-author:ItoNaokiN,
pubmed-author:KamataAkifumiA,
pubmed-author:KatsumataOsamuO,
pubmed-author:SakagamiHiroyukiH,
pubmed-author:SuzukiTatsuoT,
pubmed-author:TakedaShin'ichiS,
pubmed-author:TamakiHideakiH,
pubmed-author:WatanabeMasahikoM
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pubmed:copyrightInfo |
© 2011 The Authors. Journal of Neurochemistry © 2011 International Society for Neurochemistry.
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pubmed:issnType |
Electronic
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pubmed:volume |
116
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1122-37
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:21198641-ADP-Ribosylation Factors,
pubmed-meshheading:21198641-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:21198641-Animals,
pubmed-meshheading:21198641-Brain,
pubmed-meshheading:21198641-Carrier Proteins,
pubmed-meshheading:21198641-Cell Line, Transformed,
pubmed-meshheading:21198641-Cercopithecus aethiops,
pubmed-meshheading:21198641-Dystrophin,
pubmed-meshheading:21198641-Gene Expression Regulation,
pubmed-meshheading:21198641-Guanine Nucleotide Exchange Factors,
pubmed-meshheading:21198641-Guanosine Triphosphate,
pubmed-meshheading:21198641-Guanylate Kinase,
pubmed-meshheading:21198641-Humans,
pubmed-meshheading:21198641-Immunoprecipitation,
pubmed-meshheading:21198641-Mice,
pubmed-meshheading:21198641-Neurons,
pubmed-meshheading:21198641-Protein Binding,
pubmed-meshheading:21198641-Proteins,
pubmed-meshheading:21198641-Synapses,
pubmed-meshheading:21198641-Transfection,
pubmed-meshheading:21198641-Two-Hybrid System Techniques,
pubmed-meshheading:21198641-Vesicular Inhibitory Amino Acid Transport Proteins
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pubmed:year |
2011
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pubmed:articleTitle |
SynArfGEF is a guanine nucleotide exchange factor for Arf6 and localizes preferentially at post-synaptic specializations of inhibitory synapses.
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pubmed:affiliation |
Department of Anatomy, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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