Linked Life Data

21195433

Source:http://linkedlifedata.com/resource/pubmed/id/21195433

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2011-2-14
pubmed:abstractText
The influence of one week treatment with the choline-containing phospholipids cytidine-5'-diphosphocholine (CDP-choline) and choline alphoscerate (L-alpha-glyceryl-phosphorylcholine) at choline-equivalent doses (CDP-choline: 325 mg/kg/day; choline alphoscerate: 150 mg/kg/day) on vesicular acetylcholine transporter (VAChT), on choline transporter (CHT) and on acetylcholine (ACh) concentrations was investigated in rat frontal cortex, striatum and cerebellum. ACh was assayed by HPLC with electrochemical detection, VAChT by Western blot, ELISA and immunohistochemistry, CHT by Western blot and immunohistochemistry. After CDP-treatment, ACh levels were slightly increased in the frontal cortex, not substantially different in the striatum, and reduced significantly in the cerebellum compared to controls. Choline alphoscerate stimulated significantly the neurotransmitter concentration in the frontal cortex, however, the levels were similar to the controls in both the striatum and cerebellum. In comparison to the controls, VAChT expression following either CDP-choline or choline alphoscerate treatment, was enhanced greatly in the striatum and cerebellum. Also, ELISA measurements for VAChT showed significant increases in all choline alphoscerate treated brain areas. In contrast, in the CDP-choline treated rats the vesicular transporter amount was greater than the control only in the striatum. The cholinergic presynaptic transporters VAChT and CHT play a relevant role in sustaining new ACh synthesis and release. To sum up, CDP-choline and choline alphoscerate stimulated to a different extent the expression of VAChT and CHT primarily in a cognitive area such as frontal cortex. In the lack of novel therapeutic strategies, safe compounds developed since a long time such as the choline-containing phospholipids investigated would merit to be further investigated by new and adequate clinical studies. This for assessing their place if any in pharmacotherapy of dementia disorders characterized by diminished cholinergic tone.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1878-5883
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
302
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
49-57
pubmed:meshHeading
pubmed-meshheading:21195433-Animals, pubmed-meshheading:21195433-Blotting, Western, pubmed-meshheading:21195433-Brain Chemistry, pubmed-meshheading:21195433-Carrier Proteins, pubmed-meshheading:21195433-Choline, pubmed-meshheading:21195433-Chromatography, High Pressure Liquid, pubmed-meshheading:21195433-Cytidine Diphosphate Choline, pubmed-meshheading:21195433-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:21195433-Glycerylphosphorylcholine, pubmed-meshheading:21195433-Immunohistochemistry, pubmed-meshheading:21195433-Membrane Transport Proteins, pubmed-meshheading:21195433-Nootropic Agents, pubmed-meshheading:21195433-Parasympathetic Nervous System, pubmed-meshheading:21195433-Phospholipids, pubmed-meshheading:21195433-Rats, pubmed-meshheading:21195433-Rats, Wistar, pubmed-meshheading:21195433-Reference Standards, pubmed-meshheading:21195433-Synaptic Transmission, pubmed-meshheading:21195433-Vesicular Acetylcholine Transport Proteins
pubmed:year
2011
pubmed:articleTitle
Effect of choline-containing phospholipids on brain cholinergic transporters in the rat.
pubmed:affiliation
Anatomia Umana, Scienze del Farmaco e dei Prodotti della Salute, Università di Camerino, 62032 Camerino, Italy. khosrow.tayebati@unicam.it
pubmed:publicationType
Journal Article