Linked Life Data

21192913

Source:http://linkedlifedata.com/resource/pubmed/id/21192913

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-3-7
pubmed:abstractText
The tissue distribution of folate in its numerous coenzyme forms may influence the development of disease at different sites. For instance, the susceptibility of human colonic mucosa to localized folate deficiency may predispose to the development of colorectal cancer. We report a sensitive and robust ultra high-performance liquid chromatography (UHPLC) tandem mass spectrometry method for quantifying tissue H(4)folate, 5-CH(3)-H(4)folate, 5-CHO-H(4)folate, folic acid, and 5,10-CH(+)-H(4)folate concentration. Human colonic mucosa (20-100mg) was extracted using lipase and conjugase enzyme digestion. Rapid separation of analytes was achieved on a UHPLC 1.9-?m C18 column over 7 min. Accurate quantitation was performed using stable isotopically labeled ((13)C(5)) internal standards. The instrument response was linear over physiological concentrations of tissue folate (R(2)>0.99). Limits of detection and quantitation were less than 20 and 30 fmol on column, respectively, and within- and between-run imprecision values were 6-16%. In colonic mucosal samples from 73 individuals, the average molar distribution of folate coenzymes was 58% 5-CH(3)-H(4)folate, 20% H(4)folate, 18% formyl-H(4)folate (sum of 5-CHO-H(4)folate and 5,10-CH(+)-H(4)folate), and 4% folic acid. This assay would be useful in characterizing folate distribution in human and animal tissues as well as the role of deregulated folate homeostasis on disease pathogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1096-0309
pubmed:author
pubmed:copyrightInfo
Copyright © 2010 Elsevier Inc. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
411
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
210-7
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Quantitative analysis of tissue folate using ultra high-performance liquid chromatography tandem mass spectrometry.
pubmed:affiliation
Prince of Wales Clinical School and Adult Cancer Program, Lowy Cancer Research Centre, University of New South Wales, Sydney, NSW 2052, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't