2117396

pubmed:Citation

2

Follicular dendritic cells (FDC) from axillary lymphoid tissue of a patient with acquired immune deficiency syndrome (AIDS) were analyzed for the presence of gag and env proteins and env mRNA of human immunodeficiency virus type-1 (HIV-1), both in a purified FDC suspension and on frozen sections. Isolated cells with morphologic and immunocytochemical features of FDC expressed HIV-1 core (gag) proteins p15, p17, p24, and envelope (env) protein gp41; in addition HIV-1 env mRNA was detected in some of these cells. This corresponded with intense expression of HIV-1 proteins by FDC in germinal centers in situ, and the presence of HIV-1 mRNA-positive cells in germinal follicles. These findings led us to conclude that FDC are infected and able to produce HIV-1. Such infection may contribute significantly to the destruction of the FDC network during the lymphadenopathy phase after HIV-1 infection.

eng

AIM

MEDLINE

0002-9440

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NLM

247-51

pubmed-meshheading:2117396-Acquired Immunodeficiency Syndrome, pubmed-meshheading:2117396-Adult, pubmed-meshheading:2117396-Cell Separation, pubmed-meshheading:2117396-Dendritic Cells, pubmed-meshheading:2117396-Gene Products, gag, pubmed-meshheading:2117396-HIV Antigens, pubmed-meshheading:2117396-HIV Core Protein p24, pubmed-meshheading:2117396-HIV-1, pubmed-meshheading:2117396-Humans, pubmed-meshheading:2117396-Immunohistochemistry, pubmed-meshheading:2117396-Lymph Nodes, pubmed-meshheading:2117396-Male, pubmed-meshheading:2117396-Nucleic Acid Hybridization, pubmed-meshheading:2117396-Nucleocapsid Proteins, pubmed-meshheading:2117396-RNA, Messenger, pubmed-meshheading:2117396-RNA, Viral, pubmed-meshheading:2117396-Viral Core Proteins, pubmed-meshheading:2117396-Viral Proteins, pubmed-meshheading:2117396-gag Gene Products, Human Immunodeficiency Virus

HIV-1 infection and virus production in follicular dendritic cells in lymph nodes. A case report, with analysis of isolated follicular dendritic cells.

Journal Article, Case Reports