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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2010-12-24
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pubmed:abstractText |
Interleukin (IL)-32 is a recently described proinflammatory cytokine, characterized by induction of nuclear factor (NF)-?B activation. We studied IL-32? expression in human colonic subepithelial myofibroblasts (SEMFs). Colonic SEMFs were isolated from normal human colon tissue. IL-32? protein expression was evaluated by Western blot analyses, and IL-32? mRNA expression was analyzed by real-time PCR. IL-32? mRNA was weakly expressed without a stimulus, and its expression was markedly enhanced by IL-1ß and TNF-?. IL-1ß and TNF-? enhanced intracellular accumulation of IL-32? protein, but IL-32? was not detected in supernatants. Each cytokine dose- and time-dependently induced IL-32? mRNA expression. An inhibitor of phosphatidylinositol 3-kinase (LY294002) significantly suppressed IL-1ß- and TNF-?-induced IL-32? mRNA expression, although MAPK inhibitors had no effect. Akt activation in response to these cytokines was confirmed by Western blotting. Blockade of NF-?B activation by an adenovirus expressing a stable mutant form of I?B? markedly suppressed IL-1ß- and TNF-?-induced IL-32? mRNA expression. Human colonic SEMFs expressed IL-32? in response to IL-1ß and TNF-?. IL-32? mRNA expression depends on the phosphatidylinositol 3-kinase and the NF-?B system.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/IL32 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1beta,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1791-244X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
263-8
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pubmed:meshHeading |
pubmed-meshheading:21152864-Adjuvants, Immunologic,
pubmed-meshheading:21152864-Cell Line,
pubmed-meshheading:21152864-Cells, Cultured,
pubmed-meshheading:21152864-Colon,
pubmed-meshheading:21152864-Enzyme Induction,
pubmed-meshheading:21152864-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:21152864-Humans,
pubmed-meshheading:21152864-Interleukin-1beta,
pubmed-meshheading:21152864-Interleukins,
pubmed-meshheading:21152864-Mitogen-Activated Protein Kinases,
pubmed-meshheading:21152864-Myofibroblasts,
pubmed-meshheading:21152864-NF-kappa B,
pubmed-meshheading:21152864-Phosphatidylinositol 3-Kinase,
pubmed-meshheading:21152864-Protein Kinase Inhibitors,
pubmed-meshheading:21152864-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:21152864-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:21152864-Signal Transduction,
pubmed-meshheading:21152864-Transcription Factor AP-1,
pubmed-meshheading:21152864-Tumor Necrosis Factor-alpha
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pubmed:year |
2011
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pubmed:articleTitle |
Interleukin-32? expression in human colonic subepithelial myofibroblasts.
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pubmed:affiliation |
Department of Medicine, Graduate School of Medicine, Shiga University of Medical Science, Seta Tukinowa, Otsu, Japan.
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pubmed:publicationType |
Journal Article
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