Source:http://linkedlifedata.com/resource/pubmed/id/21108050
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2011-2-21
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| pubmed:abstractText |
The objective of this study was to investigate the role of the RhoA/Rock signaling pathway in the epithelial-mesenchymal transition (EMT) of rat peritoneal mesothelial cells (RPMCs). Primary SD rat peritoneal mesothelial cells were cultured in vitro. RPMCs were randomly assigned to four groups: group A (control), group B (TGF-?1, 10 ?g/L), group C (10 ?g/L TGF-?1?+?10 ?mol/L Y-27632, an inhibitor of Rock that was pre-applied for 2 h before TGF-?1 stimulation), and group D (Y-27632 alone, 10 ?mol/L). Our results were as follows: (1) TGF-?1 stimulation elicited a robust increase in RhoA activity in a time-dependent manner; the increase was 2.57?±?0.52 times larger than the activity observed for the control group (P?<?0.05) after 10 min of stimulation. RhoA activity peaked at 1 h and was 4.35?±?0.41 times the value observed for the control group (P?<?0.05). (2) TGF-?1 up-regulated mRNA and/or protein expression of ?-SMA, vimentin, and collagen and down-regulated mRNA and protein expression of E-cadherin in RPMCs. (3) The Rock inhibitor Y-27632 effectively reduced TGF-?1-induced expression of ?-SMA, collagen, and vimentin; the mRNA levels of ?-SMA and collagen decreased by 53.8% and 55.7%, respectively, and the protein levels of ?-SMA, vimentin, and collagen decreased by 42.6%, 60.1%, and 58.1%, respectively, as compared to TGF-?1-stimulated groups (P?<?0.05). However, the Rock inhibitor Y-27632 had no effect on the level of E-cadherin. In conclusion, the RhoA/Rock signaling pathway may mediate EMT induced by TGF-?1 in rat peritoneal mesothelial cells. The RhoA/Rock pathway may be a potential therapeutic target for the treatment of peritoneal fibrosis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1543-706X
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
47
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
165-72
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| pubmed:meshHeading |
pubmed-meshheading:21108050-Animals,
pubmed-meshheading:21108050-Cadherins,
pubmed-meshheading:21108050-Cell Culture Techniques,
pubmed-meshheading:21108050-Epithelial-Mesenchymal Transition,
pubmed-meshheading:21108050-Gene Expression Profiling,
pubmed-meshheading:21108050-Male,
pubmed-meshheading:21108050-Peritoneum,
pubmed-meshheading:21108050-Rats,
pubmed-meshheading:21108050-Signal Transduction,
pubmed-meshheading:21108050-Transforming Growth Factor beta1,
pubmed-meshheading:21108050-rhoA GTP-Binding Protein
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| pubmed:year |
2011
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| pubmed:articleTitle |
Epithelial-mesenchymal transition of rat peritoneal mesothelial cells via Rhoa/Rock pathway.
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| pubmed:affiliation |
Division of Nephrology, The Third Xiangya Hospital, South Central University, Hunan, 410013, China. zhanghaoliaoqing@163.com
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| pubmed:publicationType |
Journal Article
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