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rdf:type |
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lifeskim:mentions |
umls-concept:C0016026,
umls-concept:C0017262,
umls-concept:C0027752,
umls-concept:C0027754,
umls-concept:C0033640,
umls-concept:C0085103,
umls-concept:C0085262,
umls-concept:C0087140,
umls-concept:C0185117,
umls-concept:C1280500,
umls-concept:C1419850,
umls-concept:C2911684
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pubmed:issue |
4
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pubmed:dateCreated |
1990-3-7
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pubmed:abstractText |
The role of protein kinase C (PKC) in mediating nerve growth factor (NGF) or basic fibroblast growth factor (bFGF)-stimulated SCG10 and c-fos expression as well as neurite outgrowth was studied in PC12 cells. Activators of PKC such as phorbol 12-myristate 13-acetate (PMA) or 1-oleoyl 2-acetyl glycerol mimicked the stimulatory effect of NGF and bFGF on SCG10 mRNA levels. Induction involved a protein synthesis-dependent mechanism and was maximal within 12-24 h of exposure. Chronic treatment of the cells with PMA for up to 8 days resulted in a substantial decrease (approximately 90%) in total PKC activity in the continued presence of PMA. PKC depletion did not affect NGF- or bFGF-stimulated SCG10 mRNA induction and bFGF-stimulated c-fos mRNA induction. However, NGF-stimulated c-fos mRNA induction was attenuated. In addition, induction of neurite outgrowth was not abolished in PKC-depleted cells. The results imply that PKC is not involved in NGF- and bFGF-stimulated SCG10 mRNA induction and neurite outgrowth. Furthermore, while the effect of bFGF on c-fos mRNA induction is PKC-independent, that of NGF is mediated by PKC-dependent and -independent pathways.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-oleoyl-2-acetylglycerol,
http://linkedlifedata.com/resource/pubmed/chemical/Diglycerides,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Phorbol 12,13-Dibutyrate,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
265
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2257-61
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2105318-Adrenal Gland Neoplasms,
pubmed-meshheading:2105318-Animals,
pubmed-meshheading:2105318-Axons,
pubmed-meshheading:2105318-Cell Line,
pubmed-meshheading:2105318-Diglycerides,
pubmed-meshheading:2105318-Fibroblast Growth Factors,
pubmed-meshheading:2105318-Gene Expression Regulation,
pubmed-meshheading:2105318-Nerve Growth Factors,
pubmed-meshheading:2105318-Pheochromocytoma,
pubmed-meshheading:2105318-Phorbol 12,13-Dibutyrate,
pubmed-meshheading:2105318-Protein Kinase C,
pubmed-meshheading:2105318-Protein-Tyrosine Kinases,
pubmed-meshheading:2105318-Proto-Oncogene Proteins,
pubmed-meshheading:2105318-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:2105318-Proto-Oncogenes,
pubmed-meshheading:2105318-RNA, Messenger,
pubmed-meshheading:2105318-Rats,
pubmed-meshheading:2105318-Tetradecanoylphorbol Acetate,
pubmed-meshheading:2105318-Transcription, Genetic
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pubmed:year |
1990
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pubmed:articleTitle |
Effect of nerve growth factor and fibroblast growth factor on SCG10 and c-fos expression and neurite outgrowth in protein kinase C-depleted PC12 cells.
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pubmed:affiliation |
Department of Biochemistry, Tel Aviv University, Ramat Aviv, Israel.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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