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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1990-2-6
|
| pubmed:abstractText |
Tumor necrosis factor alpha (TNF-alpha)/cachectin is a monocyte/macrophage-derived cytokine implicated as a proximal mediator of many of the catastrophic host responses to infection or endotoxin. However, circulating levels of TNF-alpha/cachectin have only been episodically detected in hospitalized patients with life-threatening bacterial infections. In the present report, increased quantities of immune-reactive TNF-alpha/cachectin were recovered from the livers of rats 3 days following a lethal burn and infection. Two species of TNF-alpha/cachectin were detected, one of approximately 29 kd and the other 17 kd, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In murine peritoneal macrophages and rat Kupffer cells stimulated in vitro with endotoxin, a 29-kd cell-associated and 17-kd secreted form were also detected. We conclude that the increased appearance in vivo of a 29-kd form of TNF-alpha/cachectin from the livers of lethally burned and infected rats represents a novel cell-associated form of the protein.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0004-0010
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
125
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
79-84; discussion 85
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2104745-Animals,
pubmed-meshheading:2104745-Blotting, Western,
pubmed-meshheading:2104745-Burns,
pubmed-meshheading:2104745-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:2104745-Female,
pubmed-meshheading:2104745-Kupffer Cells,
pubmed-meshheading:2104745-Liver,
pubmed-meshheading:2104745-Macrophages,
pubmed-meshheading:2104745-Male,
pubmed-meshheading:2104745-Mice,
pubmed-meshheading:2104745-Mice, Inbred C57BL,
pubmed-meshheading:2104745-Molecular Weight,
pubmed-meshheading:2104745-Pseudomonas Infections,
pubmed-meshheading:2104745-Pseudomonas aeruginosa,
pubmed-meshheading:2104745-Rats,
pubmed-meshheading:2104745-Rats, Inbred Strains,
pubmed-meshheading:2104745-Sodium Dodecyl Sulfate,
pubmed-meshheading:2104745-Tumor Necrosis Factor-alpha
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Identification of a novel tumor necrosis factor alpha/cachectin from the livers of burned and infected rats.
|
| pubmed:affiliation |
Laboratory of Surgical Metabolism, New York, Hospital-Cornell University Medical College, NY 10021.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|