Source:http://linkedlifedata.com/resource/pubmed/id/20975848
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
2010-10-26
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| pubmed:abstractText |
Formaldehyde fixation and paraffin-embedding remains the most widely used technique for processing cancer tissue specimens for pathologic examination, the study of tissue morphology, and archival preservation. However, formaldehyde penetration and fixation is a slow process, requiring a minimum of 15 hr for routine processing of pathology samples. Routinely fixed samples often have a well-fixed outer rim, with a poorly-fixed inner core of tissue. In this study, we show that the application of elevated pressure up to 15,000 psi improves the rate of formaldehyde fixation by approximately 5 to 7-fold while preserving the tissue morphology of porcine liver. The tissue also exhibited much more uniform formaldehyde penetration after 30-60 min incubation under elevated pressure than samples fixed for the same length of time at atmospheric pressure.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:issn |
1837-9664
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
1
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
178-83
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| pubmed:year |
2010
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| pubmed:articleTitle |
Elevated Pressure Improves the Rate of Formalin Penetration while Preserving Tissue Morphology.
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| pubmed:affiliation |
1. Department of Biophysics, Armed Forces Institute of Pathology, Rockville, Maryland, USA;
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| pubmed:publicationType |
Journal Article
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