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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
2011-6-24
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pubmed:abstractText |
The receptor (CXCR4) for the stromal-derived factor-1 (SDF1) and the urokinase-receptor (uPAR) are up-regulated in various tumors. We show that CXCR4-transfected cells migrate toward SDF1 on collagen (CG) and do not on vitronectin (VN). Co-expression of cell-surface uPAR, which is a VN receptor, impairs SDF1-induced migration on CG and allows migration on VN. Blocking fMLP receptors (fMLP-R), alpha-v integrins or the uPAR region capable to interact with fMLP-Rs, impairs migration of uPAR/CXCR4-transfected cells on VN and restores their migration on CG. uPAR co-expression also reduces the adherence of CXCR4-expressing cells to various components of the extracellular matrix (ECM) and influences the partitioning of beta1 and alpha-v integrins to membrane lipid-rafts, affecting ECM-dependent signaling. uPAR interference in CXCR4 activity has been confirmed in cells from prostate carcinoma. Our results demonstrate that uPAR expression regulates the adhesive and migratory ability of CXCR4-expressing cells through a mechanism involving fMLP receptors and alpha-v integrins.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL12,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/FPR3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Integrin alpha5beta1,
http://linkedlifedata.com/resource/pubmed/chemical/MAPK1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CXCR4,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Formyl Peptide,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Urokinase Plasminogen...,
http://linkedlifedata.com/resource/pubmed/chemical/Vitronectin
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1420-9071
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pubmed:author |
pubmed-author:AlfanoDanielaD,
pubmed-author:BifulcoKatiaK,
pubmed-author:CarrieroMaria VincenzaMV,
pubmed-author:La PennaClaudioC,
pubmed-author:MontuoriNunziaN,
pubmed-author:PesapaneAdaA,
pubmed-author:RagnoPiaP,
pubmed-author:RossiFrancesca WandaFW,
pubmed-author:RossiGuidoG,
pubmed-author:SalzanoSalvatoreS,
pubmed-author:VisconteValeriaV
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pubmed:issnType |
Electronic
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2453-67
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pubmed:meshHeading |
pubmed-meshheading:20972812-Blotting, Western,
pubmed-meshheading:20972812-Cell Adhesion,
pubmed-meshheading:20972812-Cell Line, Tumor,
pubmed-meshheading:20972812-Cell Membrane,
pubmed-meshheading:20972812-Cell Movement,
pubmed-meshheading:20972812-Chemokine CXCL12,
pubmed-meshheading:20972812-Collagen,
pubmed-meshheading:20972812-Enzyme Activation,
pubmed-meshheading:20972812-HEK293 Cells,
pubmed-meshheading:20972812-Humans,
pubmed-meshheading:20972812-Integrin alpha5beta1,
pubmed-meshheading:20972812-Membrane Microdomains,
pubmed-meshheading:20972812-Microscopy, Confocal,
pubmed-meshheading:20972812-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:20972812-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:20972812-RNA Interference,
pubmed-meshheading:20972812-Receptor Cross-Talk,
pubmed-meshheading:20972812-Receptors, CXCR4,
pubmed-meshheading:20972812-Receptors, Formyl Peptide,
pubmed-meshheading:20972812-Receptors, Urokinase Plasminogen Activator,
pubmed-meshheading:20972812-Transfection,
pubmed-meshheading:20972812-Vitronectin
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pubmed:year |
2011
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pubmed:articleTitle |
The cross-talk between the urokinase receptor and fMLP receptors regulates the activity of the CXCR4 chemokine receptor.
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pubmed:affiliation |
Department of Cellular and Molecular Biology and Pathology, Federico II University, Naples, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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