Linked Life Data

20972399

Source:http://linkedlifedata.com/resource/pubmed/id/20972399

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
43
pubmed:dateCreated
2010-10-25
pubmed:abstractText
Most life forms exhibit daily rhythms in cellular, physiological and behavioral phenomena that are driven by endogenous circadian (?24 hr) pacemakers or clocks. Malfunctions in the human circadian system are associated with numerous diseases or disorders. Much progress towards our understanding of the mechanisms underlying circadian rhythms has emerged from genetic screens whereby an easily measured behavioral rhythm is used as a read-out of clock function. Studies using Drosophila have made seminal contributions to our understanding of the cellular and biochemical bases underlying circadian rhythms. The standard circadian behavioral read-out measured in Drosophila is locomotor activity. In general, the monitoring system involves specially designed devices that can measure the locomotor movement of Drosophila. These devices are housed in environmentally controlled incubators located in a darkroom and are based on using the interruption of a beam of infrared light to record the locomotor activity of individual flies contained inside small tubes. When measured over many days, Drosophila exhibit daily cycles of activity and inactivity, a behavioral rhythm that is governed by the animal's endogenous circadian system. The overall procedure has been simplified with the advent of commercially available locomotor activity monitoring devices and the development of software programs for data analysis. We use the system from Trikinetics Inc., which is the procedure described here and is currently the most popular system used worldwide. More recently, the same monitoring devices have been used to study sleep behavior in Drosophila. Because the daily wake-sleep cycles of many flies can be measured simultaneously and only 1 to 2 weeks worth of continuous locomotor activity data is usually sufficient, this system is ideal for large-scale screens to identify Drosophila manifesting altered circadian or sleep properties.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/20972399-10707978, http://linkedlifedata.com/resource/pubmed/commentcorrection/20972399-10762032, http://linkedlifedata.com/resource/pubmed/commentcorrection/20972399-11675495, http://linkedlifedata.com/resource/pubmed/commentcorrection/20972399-15262411, http://linkedlifedata.com/resource/pubmed/commentcorrection/20972399-15817324, http://linkedlifedata.com/resource/pubmed/commentcorrection/20972399-16085487, http://linkedlifedata.com/resource/pubmed/commentcorrection/20972399-16938867, http://linkedlifedata.com/resource/pubmed/commentcorrection/20972399-18419319, http://linkedlifedata.com/resource/pubmed/commentcorrection/20972399-18593878, http://linkedlifedata.com/resource/pubmed/commentcorrection/20972399-19014079, http://linkedlifedata.com/resource/pubmed/commentcorrection/20972399-19874792, http://linkedlifedata.com/resource/pubmed/commentcorrection/20972399-20041201, http://linkedlifedata.com/resource/pubmed/commentcorrection/20972399-5002428, http://linkedlifedata.com/resource/pubmed/commentcorrection/20972399-8223268
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1940-087X
pubmed:author
pubmed:issnType
Electronic
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2010
pubmed:articleTitle
Assaying locomotor activity to study circadian rhythms and sleep parameters in Drosophila.
pubmed:affiliation
Center for Advanced Biotechnology and Medicine, Rutgers University, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural, Video-Audio Media